| 基于磁性金属有机框架分离的纳米金比色法检测单核细胞增生李斯特菌 |
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| 引用本文： | 杜娟,陈鑫,刘楷,刘佳蕾,骆鹏杰,梁栋,白艳红.基于磁性金属有机框架分离的纳米金比色法检测单核细胞增生李斯特菌[J].食品科学,2023,44(6):360-367. |
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| 作者姓名： | 杜娟 陈鑫 刘楷 刘佳蕾 骆鹏杰 梁栋 白艳红 |
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| 作者单位： | （1.郑州轻工业大学食品与生物工程学院，河南省冷链食品质量与安全控制重点实验室，河南 郑州 450001；2.国家食品安全风险评估中心 国家卫生健康委员会食品安全风险评估重点实验室，中国医学科学院创新单元（2019RU014号），北京 100022；3.国家食品安全风险评估中心应用营养一室，北京 100022） |
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| 基金项目： | 河南省重大公益专项（201300110100） |
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| 摘 要： | 以磁性金属有机框架复合材料作为捕获探针，免疫功能化纳米金作为信号探针，建立一种纳米金比色法快速检测单核细胞增生李斯特菌的方法。结果表明：在最适条件下，单核细胞增生李斯特菌的可视化检出限为1.2×103 CFU/m L，紫外光谱检测结果在1.2×101～1.2×108 CFU/mL范围内有良好的线性关系(Y=0.519-0.043X,R2=0.978)，检出限低至0.45 CFU/mL，各浓度梯度的批内变异系数在0.31%～1.30%之间。将单核细胞增生李斯特菌接种到鸡胸肉上进行检测，其加标回收率在91.1%～108.7%之间，变异系数不高于7.4%，且结果与平板计数法一致。本方法具有准确、灵敏、快速等特点，在食源性致病菌检测方面具有较好的应用前景。
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| 关 键 词： | 单核细胞增生李斯特菌 纳米金 比色法 磁分离 |
Highly Sensitive Colorimetric Detection of Listeria monocytogenes by Using Gold Nanoparticles Based on Magnetic Metal-Organic Framework Separation |
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| DU Juan,CHEN Xin,LIU Kai,LIU Jialei,LUO Pengjie,LIANG Dong,BAI Yanhong.Highly Sensitive Colorimetric Detection of Listeria monocytogenes by Using Gold Nanoparticles Based on Magnetic Metal-Organic Framework Separation[J].Food Science,2023,44(6):360-367. |
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| Authors: | DU Juan CHEN Xin LIU Kai LIU Jialei LUO Pengjie LIANG Dong BAI Yanhong |
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| Affiliation: | (1. College of Food and Bioengineering, Henan Key Laboratory of Cold Chain Food Quality and Safety Control, Zhengzhou University of Light Industry, Zhengzhou 450001, China; 2. NHC Key Laboratory of Food Safety Risk Assessment, Chinese Academy of Medical Science Research Unit (2019RU014), China National Center for Food Safety Risk Assessment, Beijing 100022, China; 3. Applied Nutrition I, China National Center for Food Safety Risk Assessment, Beijing 100022, China) |
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| Abstract: | In this study, magnetic metal organic framework composites as capture probe and immunofunctionalized gold nanoparticles as signal probe were used to develop a rapid colorimetric method for the detection of Listeria monocytogenes. Under optimal conditions, the visual detection limit for L. monocytogenes was 1.2 × 103 CFU/mL. The results of ultraviolet spectroscopy showed a good linear relationship in the range of 1.2 × 101?1.2 × 108 CFU/mL (Y = 0.519 ? 0.043X, R2 = 0.978), the detection limit was as low as 0.45 CFU/mL, and the intra-batch coefficient of variation of all concentration gradients was between 0.31% and 1.30%. The recoveries for spiked chicken breast were 91.1%?108.7% with coefficient of variation less than 7.4%, and the result was consistent with that of the plate counting method. In conclusion, the proposed assay is rapid, accurate sensitive, and highly applicable to the detection of foodborne pathogens. |
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| Keywords: | Listeria monocytogenes gold nanoparticles colorimetric assay magnetic separation |
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