气相色谱法测定脂肪酸含量判定山茶油纯度

利用气相色谱法,对山茶油掺入大豆油、菜籽油、玉米油和葵花籽油的掺伪油进行脂肪酸组成分析。结果表明:油酸、亚油酸和亚麻酸可作为鉴别山茶油中掺伪大豆油和菜籽油的特征脂肪酸,棕榈酸、油酸和亚油酸可作为鉴别山茶油中掺伪玉米油和葵花籽油的特征脂肪酸;回归预测模型相关系数(R^2)较高(> 0. 99),可分别检出掺伪量4%的大豆油和菜籽油,掺伪量8%的玉米油和葵花籽油,回收率在96. 56%~112. 88%之间。该方法灵敏度高,定量准确,可为掺伪山茶油纯度鉴别及调和山茶油配比的定量分析提供科学依据。

udgement of purity of oil-tea camellia seed oil by determining fatty acidcontent with gas chromatography

The fatty acid composition of oil-tea camellia seed oil adulterated with soybean oil, rapeseed oil, corn oil or sunflower seed oil was analyzed by gas chromatography. The results showed that oleic acid, linoleic acid and linolenic acid could be used as the characteristic fatty acids in the identification of soybean oil and rapeseed oil, while palmitic acid, oleic acid and linoleic acid could be used as the characteristic fatty acids to identify corn oil and sunflower seed oil. The correlation coefficient of regression prediction model (R2) was higher (>0.99). The regression prediction model could be used to identify 4% of soybean oil or rapeseed oil as well as 8% of corn oil or sunflower seed oil, with recoveries ranging from 96.56% to 112.88%. The established method was sensitive and accurate, and could provide scientific basis for purity identification of blend oil-tea camellia seed oil and quantitative analysis of adulterated oil-tea camellia seed oil.