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| 离子对反相高效液相色谱法测定猪硫酸软骨素的含量 | |
| 引用本文： | 陆钊,杨传禹,刘晶晶,赵野,尹涛,杨铮志.离子对反相高效液相色谱法测定猪硫酸软骨素的含量[J].广东化工,2011,38(10):141-142. |
| 作者姓名： | 陆钊 杨传禹 刘晶晶 赵野 尹涛 杨铮志 |
| 作者单位： | 1. 吉林医药学院药学院化学教研室,吉林吉林,132013 2. 吉林医药学院药学院08级临床药学本科班,吉林吉林,132013 3. 吉林医药学院口腔影像系09级本科班,吉林吉林,132013 |
| 摘 要： | 目的：采用离子对反相高效液相色谱法测定猪硫酸软骨素的含量。方法：采用Inertsit-ODS-SP色谱柱（4.6×150 mm,5μm）,流动相：乙腈—0.006 mol.L-1辛烷基磺酸钠溶液（15：85）,磷酸调pH至3.5,流速：0.8 mL.min-1,测定波长：195 nm,进样量：20μL。结果：该方法在质... |
| 关 键 词： | 猪硫酸软骨素 离子对反相高效液相色谱 外标法 测定 |
The Determination of the Content of Chondroitin Sulfate Porcine with Ion-pair Reversed-phase High Performance Iiquid Chromatography |
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| Lu Zhao,Yang Chuanyu,Liu Jingjing,Zhao Ye,Yin Tao,Yang Zhengzhi.The Determination of the Content of Chondroitin Sulfate Porcine with Ion-pair Reversed-phase High Performance Iiquid Chromatography[J].Guangdong Chemical Industry,2011,38(10):141-142. | |
| Authors: | Lu Zhao Yang Chuanyu Liu Jingjing Zhao Ye Yin Tao Yang Zhengzhi |
| Affiliation: | Lu Zhao1,Yang Chuanyu2,Liu Jingjing2,Zhao Ye2,Yin Tao2,Yang Zhengzhi3(1.Jilin Medical College Department of Chemistry of Institute of Pharmacy,Jilin 1320132,2.Jilin Medical College 08 Clinical Pharmacy Class of Institute of Pharmacy,Jilin 132013,3.Jilin Medical College Department of Oral Imaging,China) |
| Abstract: | Purpose： Determine the content of chondroitin sulfate porcine with ion-pair reversed-phase high performance liquid chromatography.Method： Use Inertsit-ODS-SP chromatographic column（4.6×150 mm,5 μm）,mobile phase： Acetonitrile-0.006 mol·L-1 octanesulfonic acid sodium salt solution（15：85）,phosphoric acid pH 3.5,velocity of flow： 0.8 mL·min-1,range of wavelength： 195 nm,inpouring： 20 μL.Result： The method has a good linear relationship within the range of a mass concentration of 0.01~0.08 mg·mL-1,r=0.9998,recovery 99.25 %,RSD=1.51 %.Conclusion： The method is precise and exact,and it can be used to determine the content of chondroitin sulfate porcine.Chromatography external standard method determination. |
| Keywords: | chondroitin sulfate porcine ion-pair reversed-phase high performance liquid chromatography external standard method determination |
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