| 用辣根过氧化物酶生物传感器测定啤酒中的过氧化氢 |
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| 引用本文： | 冯东,李雪梅,王丙莲,李大海,刘仲汇.用辣根过氧化物酶生物传感器测定啤酒中的过氧化氢[J].酿酒科技,2011(12):37-39. |
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| 作者姓名： | 冯东 李雪梅 王丙莲 李大海 刘仲汇 |
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| 作者单位： | 山东省科学院生物研究所,山东济南,250014 |
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| 基金项目： | 基金项目:山东省科技攻关项目，山东省自然科学基金，济南市青年科技明星计划，山东省博士后专项创新基金 |
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| 摘 要： | 用一种新型的壳聚糖(CS)/聚乙烯吡咯烷酮(PVP)复合膜固定辣根过氧化物酶(HRP),以乙二醛作交联剂,二茂铁(Fc)作媒介体,制备过氧化氢生物传感器。采用循环伏安法对该传感器的性能进行研究。结果表明,传感器在H2O2浓度为3.00×10-8～3.00×10-4 mol/L的范围内具有良好的线性相关性。使用SBA-50生物传感分析仪测定啤酒中的H2O2,回收率为97.6%～101.8%。
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| 关 键 词： | 啤酒 过氧化氢 辣根过氧化物酶 生物传感器 |
Determination of Hydrogen Peroxide in Beer by Immobilized Horseradish Peroxidase Biosensor |
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| FENG Dong,LI Xuemei,WANG Binglian,LI Dahai,and LIU Zhonghui.Determination of Hydrogen Peroxide in Beer by Immobilized Horseradish Peroxidase Biosensor[J].Liquor-making Science & Technology,2011(12):37-39. |
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| Authors: | FENG Dong LI Xuemei WANG Binglian LI Dahai and LIU Zhonghui |
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| Affiliation: | 1,2(1.Biology Research Institute of Shandong Academy of Sciences,Jinan,Shandong 250014;2.Key Lab for Biosensors of Shandong Province,Jinan,Shandong 250014,China) |
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| Abstract: | Hydrogen peroxide biosensor has been developed by immobilizing horseradish peroxidase （HRP） in a new-type chitosan （CS） /polyvinylpyrrolidone （PVP） hybrid film with glyoxal as a crosslinker and ferrocene as a mediator. The performance of such biosensor was studied by means of cyclic voltammetry. The results showed that the biosensor had a good linear relationship to H2O2 in the range of 3.00× 10-8 mol/L 3.00- 10-4 mol/L. SBA-50 biosensor was applied to determine H2O2 in beer samples with its recovery between 97.6 %-101.8 %. |
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| Keywords: | beer hydrogen peroxide horseradish peroxidase biosensor |
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