Bioassay-guided isolation and identification of antifungal components from propolis against Penicillium italicum |
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| Authors: | SZ Yang LT Peng XJ Su F Chen YJ Cheng G Fan SY Pan |
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| Affiliation: | 1. College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, PR China;2. Key Laboratory for Crop Germplasm Innovation and Utilization of Hunan Province, Hunan Agricultural University, Changsha 410128, PR China;3. Department of Food Science and Human Nutrition, Clemson University, Clemson SC 29634, USA;4. College of Horticulture and Forestry, Huazhong Agricultural University, Wuhan 430070, PR China |
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| Abstract: | The present study was aimed at identification of antifungal components against Penicillium italicum from Chinese propolis with bioassay-guided fractionation technique. Propolis ethanolic extract (PEE) was separated and purified by liquid–liquid extraction and thin layer chromatography (TLC) and the most active band was subjected to HPLC–MS/MS to identify the antifungal compounds. The results showed PEE and its fractions had strong antifungal activity against P. italicum. Among the fractions of PEE partitioned by petroleum ether, ethyl acetate, n-butanol and water, ethyl acetate fraction (E-Fr) exhibited the most effective activity against P. italicum. Further bioautographic TLC assay showed Band I, with Rf value of 0.70, had an inhibitive zone, which showed the strongest antifungal activity and completely inhibited the growth of P.italicum at 200 mg/L. Bioactive components found in Band I were further identified as pinobanksin, pinocembrine, chrysin and galangin. This study exhibited Chinese propolis and its main flavonoids was potential natural alternatives for the control of citrus blue mould caused by P.italicum. |
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| Keywords: | Penicillium italicum Propolis Bioautographic assay HPLC&ndash MS/MS Antifungal activity |
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