QuEChERS净化—气相色谱—质谱法测定食用植物油中4种合成抗氧化剂

建立了一种QuEChERS净化—气相色谱—质谱(GC-MS)快速测定7类食用植物油中4种合成抗氧化剂(BHT、BHA、TBHQ、Ionox-100)的方法。样品经乙腈超声提取后,运用QuEChERS技术净化,采用DB-35MS毛细管色谱柱(30 m×0.250 mm×0.25μm)分离,选择离子监测(SIM)模式进行测定,外标法定量。结果表明:各合成抗氧化剂在0.5~20.0μg/mL内线性关系良好,其相关系数R 2均>0.999,相对标准偏差RSD为0.4%~4.5%,检出限为0.05 mg/kg,加标回收率为84.1%~108.0%,采用质控样品进行验证,结果均为满意。该方法适用于食用植物油中抗氧化剂含量的快速筛查和测定。

Determination of four synthetic antioxidants in edible vegetable oil by gas chromatography-mass spectrometry coupled with QuEChERS

To establish a gas chromatography-mass spectrometry(GC-MS)method for the rapid determination of four synthetic antioxidants(BHT,BHA,TBHQ,and Ionox-100)in seven kinds of edible vegetable oils by QuEChERS assisted with ultrasonic extraction and purification.After ultrasonic extraction with acetonitrile,the samples were purified by QuEChERS technology,DB-35MS capillary column(30 m×0.250 mm×0.25μm)was used for separation of analytes and ion monitoring(SIM)mode was selected for determination.The analytes were quantified by external standard method.The results indicated that the linear range of synthetic antioxidants was 0.5~20.0μg/mL,and the correlation coefficient(R 2)was greater than 0.999,the relative standard deviation(RSD)was 0.4%~4.5%,and the detection limit was 0.05 mg/kg,with the recovery 84.1%~108.0%.The results of quality control samples used for verification were also satisfactory.The method is suitable for rapid screening and determination of antioxidant content in edible vegetable oil.