| 具有自由基清除活性的鱿鱼皮胶原蛋白肽酶解工艺研究 |
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| 引用本文： | 许丹,杨会成,郑斌,丁国芳.具有自由基清除活性的鱿鱼皮胶原蛋白肽酶解工艺研究[J].中国酿造,2012,31(6):79-82. |
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| 作者姓名： | 许丹 杨会成 郑斌 丁国芳 |
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| 作者单位： | 1. 浙江海洋学院食品医药学院,浙江舟山,316000
2. 浙江省海洋开发研究院,浙江舟山,316100 |
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| 基金项目： | 浙江省厅市会商项目（2011C02003）; 舟山市重大海洋类项目（2011C22059）; 2011年浙江省大学生技创新（新苗人才） |
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| 摘 要： | 以蛋白水解度和羟自由基清除率为指标,采用胰蛋白酶法提取秘鲁鱿鱼皮中的胶原蛋白肽,并通过单因素试验和正交试验优化了鱼皮胶原蛋白肽的制备工艺条件,同时测定了鱼皮的基本组成成分.结果表明,加酶量5％,酶解时间7h,酶解温度50℃,pH值8.0,酶解效果最好.此条件下,鱿鱼皮胶原蛋白肽的水解度为26.1％,羟自由基清除率为83.3％,在医用保健食品和抗衰老美容护肤产品上具有很好的实用价值.
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| 关 键 词： | 胰蛋白酶 鱿鱼皮 胶原蛋白肽 羟自由基 |
Enzymolysis technology optimization for production of collagen peptides from squid skin with free radical scavenging activity |
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| XU Dan
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YANG Huicheng
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ZHENG Bin
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DING Guofang.Enzymolysis technology optimization for production of collagen peptides from squid skin with free radical scavenging activity[J].China Brewing,2012,31(6):79-82. |
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| Authors: | XU Dan YANG Huicheng ZHENG Bin DING Guofang |
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| Affiliation: | 1 School of Food and Pharmacy ＆ Medical School, Zhejiang Ocean University, Zhoushan 316000, China; 2.Zhejiang Marine Development Research Institute, Zhoushan, 316100, China) |
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| Abstract: | The collagen peptide from squid skin has been extracted by using protease based on the protein degree of hydrolysis （DH） and hydroxyl rad-icals scavenging rate. Through single factor experiment and orthogonal test, the preparation technology conditions of fish skin collagen peptide were optimized and the basic composition of squid skin was also analyzed. The results showed that the optimum addition of trypsin was 5%, the enzymoly- sis time was 7h, enzymolysis temperature was 50℃, and pH value was 8.0. Under these conditions, the maximum DH was 26. 1% and the hydroxyl radical scavenging rate was 83.3%. Squid skin collagen peptide has wide practical application value in medical healthy food and anti-aging, beauty skin care. |
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| Keywords: | trypsin squid skin collagen peptides hydroxyl free radical |
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