| 基于核酸适配体的生物传感技术检测食源性致病菌研究进展 |
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| 引用本文： | 秦铭灿,田雪晴,周俊栏,方再郗,周皓隆,高仁姣,吕明生,王淑军.基于核酸适配体的生物传感技术检测食源性致病菌研究进展[J].食品安全质量检测技术,2021,12(13):5237-5243. |
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| 作者姓名： | 秦铭灿 田雪晴 周俊栏 方再郗 周皓隆 高仁姣 吕明生 王淑军 |
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| 作者单位： | 江苏海洋大学海洋资源与环境重点实验室/海洋生物技术重点实验室,江苏海洋大学海洋资源与环境重点实验室/海洋生物技术重点实验室,江苏大学计算机科学与通信工程学院;江苏大学计算机科学与通信工程学院,江苏海洋大学海洋资源与环境重点实验室/海洋生物技术重点实验室,江苏海洋大学海洋资源与环境重点实验室/海洋生物技术重点实验室 |
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| 基金项目： | 国家重点研发计划。海洋来源高附加值产业化酶的挖掘和新产品开发(2018YFC0311106); 江苏省高等教育优势学科(PAPD); 江苏省研究生创新项目 (SJCX19-1012，ZD201918） |
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| 摘 要： | 食品供应的全球化导致食源性疾病传播快、分布广，严重威胁人类健康。病原检测需要特异性强和灵敏度高的方法。核酸适配体是可以识别并与多种类型靶标分子的单链DNA或RNA。基于核酸适配体的生物传感器特异性好、灵敏度高、易储存。为食源性致病菌快速检测提供了新的方法。本文介绍了核酸适配体的特点和筛选技术，综述了基于适配体的电化学、比色、荧光、表面增强拉曼散射和质量生物传感器技术的基本原理及其在食源性致病菌检测中的应用，以期为开发高效、精准检测食源致病菌技术提供参考。
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| 关 键 词： | 食源性致病菌 核酸适配体 生物传感器 |
| 收稿时间： | 2021/4/13 0:00:00 |
| 修稿时间： | 2021/6/11 0:00:00 |
Research progress on the detection of foodborne pathogens by biosensor based on aptamers |
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| QIN Ming-Can,TIAN Xue-Qing,ZHOU Jun-Lan,FANG Zai-Xi,ZHOU Hao-Long,GAO Ren-Jiao,LV Ming-Sheng,WANG Shu-Jun.Research progress on the detection of foodborne pathogens by biosensor based on aptamers[J].Food Safety and Quality Detection Technology,2021,12(13):5237-5243. |
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| Authors: | QIN Ming-Can TIAN Xue-Qing ZHOU Jun-Lan FANG Zai-Xi ZHOU Hao-Long GAO Ren-Jiao LV Ming-Sheng WANG Shu-Jun |
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| Affiliation: | Jiangsu Key Laboratory of Marine Bioresources and Environment/Jiangsu Key Laboratory of Marine Biotechnology,Jiangsu Ocean University,Jiangsu Key Laboratory of Marine Bioresources and Environment/Jiangsu Key Laboratory of Marine Biotechnology,Jiangsu Ocean University,College of computer science and Communication Engineering,Jiangsu University,Jiangsu Key Laboratory of Marine Bioresources and Environment/Jiangsu Key Laboratory of Marine Biotechnology,Jiangsu Ocean University,Jiangsu Key Laboratory of Marine Bioresources and Environment/Jiangsu Key Laboratory of Marine Biotechnology,Jiangsu Ocean University |
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| Abstract: | The globalization of food supply may lead to the rapid spread and wide distribution of foodborne diseases, which seriously endanger human healthy. It is required for the technology of pathogen detection with highly specific and sensitive. Aptamers are single stranded DNA or RNA molecules which can recognize and bind to target molecules. Aptamer based biosensors are more specific and sensitive, easy to store, and provide new methods for rapid detection of foodborne pathogens. This paper introduces the characteristics and screening technology of aptamers, and summarizes the basic principles of aptamer based electrochemical, colorimetric, fluorescence, surface enhanced Raman scattering and quality biosensor technology and its application in the detection of foodborne pathogens. This review provided reference for the development of fast, efficient, and accurate detection technology of foodborne pathogens. |
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| Keywords: | Foodborne pathogens Aptamer Biosensor |
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