南瓜籽甾醇对大鼠前列腺增生 及抗氧化能力的影响

将提取纯化的南瓜籽甾醇作用于丙酸睾酮诱导构建的前列腺增生大鼠模型，研究其对大鼠前列腺增生及抗氧化能力的影响。将50只雄性SD大鼠分为对照组，模型组，南瓜籽甾醇高、中、低剂量组，实验结束后分别测定其前列腺湿重、前列腺指数（PI）、前列腺酸性磷酸酶（PACP）活性及抗氧化指标中的总抗氧化能力（T-AOC）水平、超氧化物歧化酶（SOD）活性和丙二醛（MDA）含量，同时对前列腺组织形态进行切片观察。结果表明：与模型组相比，南瓜籽甾醇高剂量组的前列腺湿重、PI、PACP活性与MDA含量显著降低，并且南瓜籽甾醇中、高剂量组的T-AOC水平、SOD活性显著升高。以上指标的变化均呈现出南瓜籽甾醇高剂量组优于中剂量组的趋势。同时，与模型组相比，南瓜籽甾醇中、高剂量组大鼠前列腺组织细胞受损状况均有所改善，南瓜籽甾醇高剂量组的改善效果更明显。因此，南瓜籽甾醇在一定程度上具有改善大鼠前列腺增生及提高大鼠前列腺组织的抗氧化能力的作用。

Effect of pumpkin seed sterol on benign prostatic hyperplasia and antioxidant capacity of rats

The extracted and purified pumpkin seed sterol was used to feed the rats with benign prostate hyperplasia induced by testosterone propionate to study its effect on the benign prostate hyperplasia and antioxidant capacity of rats. 50 Male SD rats were divided into control group, model group, high, medium and low dose groups of pumpkin seed sterol. After the experiment, the prostate wet weight, prostate index (PI), and levels of prostatic acid phosphatase (PACP), total antioxidant capacity (T-AOC), superoxide dismutase (SOD) and malondialdehyde (MDA) were measured, the morphology of prostate tissue was observed simultaneously. The results showed that compared with the model group, the wet weight, PI, PACP activities, and MDA content of the high dose group of pumpkin seed sterol significantly decreased, and the T-AOC level and SOD activity in the midium and high dose groups of pumpkin seed sterol significantly increased. All of the above changes showed a trend of high dose group of pumpkin seed sterol better than medium dose group. At the same time, the prostate tissue cell damages of rats in medium and high dose groups of the pumpkin seed sterol were improved compared with the model group, and high dose group of the pumpkin seed sterol had the most obvious effect. This indicated that pumpkin seed sterol improved the prostate hyperplasia in rats to some extent and improved the antioxidant capacity of rat prostate tissue.