| 乳制品中高产双乙酰乳酸菌的筛选及鉴定 |
| |
| 引用本文： | 田玉娟,周康,韩新锋,刘书亮.乳制品中高产双乙酰乳酸菌的筛选及鉴定[J].食品科学,2015,36(3):162-165. |
| |
| 作者姓名： | 田玉娟 周康 韩新锋 刘书亮 |
| |
| 作者单位： | 四川农业大学食品学院，四川 雅安 625014 |
| |
| 摘 要： | 以四川省生鲜乳和发酵乳为原料,利用MRS培养基进行分离筛选,共分离出39株乳酸菌,对其中37株进行产双乙酰的初筛,分离出7株产量较高的菌株,最后从这7株中确定出1株产双乙酰量最高的乳酸菌YJ-110通过分光光度计法测得该菌株双乙酰的产量为23.826 mg/L。经形态学、生理生化特性和16S rDNA分子鉴定,确定该菌为嗜柠檬酸明串珠菌(Leuconostoc citreum)。
|
| 关 键 词： | 双乙酰 分离鉴定 明串珠菌 |
Isolation and Identification of High-Level Diacetyl-Producing Lactic Acid Bacteria from Milk Products |
| |
| TIAN Yujuan;ZHOU Kang;HAN Xinfeng;LIU Shuliang.Isolation and Identification of High-Level Diacetyl-Producing Lactic Acid Bacteria from Milk Products[J].Food Science,2015,36(3):162-165. |
| |
| Authors: | TIAN Yujuan;ZHOU Kang;HAN Xinfeng;LIU Shuliang |
| |
| Affiliation: | College of Food Science, Sichuan Agricultural University, Ya’an 625014, China |
| |
| Abstract: | In this study, using MRS medium, we isolated 39 strains from raw milk and fermented milk from Sichuan
province, of which 37 were found to be capable of diacetyl. Seven strains with increased diacetyl-producing ability were
selected from the 37 strains and YJ-110, a Lactobacillus strain, produced the highest yield (23.826 mg/L as measured
by spectrophotometer) of diacetyl among the seven strains. Based on morphological, physiological and biochemical
characteristics and 16S rDNA sequences, this strain was identified as Leuconostoc citreum. |
| |
| Keywords: | diacetyl isolation and identification Leuconostoc citreum |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《食品科学》浏览原始摘要信息 |
|
点击此处可从《食品科学》下载全文 |
|
