离子色谱法测定肉制品中亚硝酸盐与硝酸盐的改进研究

改进中国GB 5009.33—2010标准方法中离子色谱法测定肉制品中亚硝酸盐与硝酸盐的样品前处理条件。GB5009.33—2010标准采用滤纸过滤样品提取液除脂肪,研究发现滤纸的使用会干扰硝酸盐检测导致其结果严重偏高。本研究改用三氯甲烷除去脂肪有效地解决了该标准方法存在的弊端,同时查明了肉制品中硝酸盐普遍超标并非因企业违规使用含有硝酸盐的添加剂,而是因加工过程中使用了富含硝酸盐的香辛料、辣椒等植物性原料本底。试验证明:改进方法简便、快速、灵敏、准确,适用于肉制品中亚硝酸盐与硝酸盐的定量测定。亚硝酸盐和硝酸盐在所测浓度范围内与峰面积呈良好的线性关系,加标回收率为94.5%~100.5%,相对标准偏差(RSD)为0.70%~3.5%。

Improved research for determination of nitrite and nitrate in meat products by Ion Chromatography

Improvement of sample pretreatment conditions of ion chromatography method of national standard methods to determinate the nitrite and nitrate in meat products. The national standard methods employ filter paper to remove the fat in the sample extract. However, it is found the usage of filter paper can disturb the detection of nitrate, and cause serious high detection results. In this study, using chloroform to remove the fat can efficiently solve the disadvantages exist in the national standard methods. The generally exceed the standard of nitrate in the meats is not caused by the enterprise of illegal use of additives containing nitrate,and it is caused by the usage of spice, which is rich in nitrate, and peppers etc. plant material in the process of machining. Experiments proved that the improved method is simple, rapid, sensitive, accurate and suitable for the quantitative determination of nitrite and nitrate in meat products. Nitrite and nitrate in the measured concentration range showed a good linear relationship with the peak area. The recoveries were between 94.5% and 100.5%. The relative standard deviations were between 0.70% and 3.5%.