长寿人群饮食中膳食纤维对C57BL/6小鼠的抗氧化效果

Antioxidant Effect of Dietary Fiber in the Diet of Longevity Population on C57BL/6 Mice

In order to study the antioxidant effect of dietary fiber in long-lived people in Bemba, Guangxi, six-week-old healthy C57BL/6 mice were selected as study subjects. Seven kinds of dietary fiber (DF), which the most representative of longevity population in Bama, Guangxi, were selected, including banana, navel orange, pumpkin seedling, sweet potato leaf, water spinach, Ixeris sonchifolia and agrocybe chaxingu, and 10 feeding patterns were designed. Mice were fed with these 10 patterns for 4 weeks. The contents of malondialdehyde (MDA) and lipofuscin (Lip), activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) and total antioxidant capacity (T-AOC) in serum and liver tissues of the tested mice were determined. The results showed that except for Ixeris sonchifolia dietary fiber, the addition of single dietary fiber had no significant effect on the weight gain of mice. In a certain range, the larger the addition of compound dietary fiber, the slower the weight gain rate of mice. Among them, the heart coefficients of mice in the 7.5% and 15% compound dietary fiber addition groups were increased by 7.22 % (P<0.05) and 6.92 % (P<0.05), respectively. The spleen coefficients of mice were increased by 10.00%, 15.00% by adding pumpkin seedling dietary fiber isolate and 30% composite dietary fiber isolate. The dietary fiber isolates in each group could effectively reduce the levels of MDA and lipofuscin Lip in serum and liver of mice , and improve the activities of SOD, GSH-Px and T-AOC in serum and liver of mice. Comprehensive analysis shows that adding compound dietary fiber to the diet of mice has stronger antioxidant stress and better antioxidant effect.