富含益生菌杂豆粉工艺条件优化及货架期预测

以绿豆为原料制备富含益生菌绿豆粉，对其制备工艺条件进行优化，并验证了该制备工艺条件对不同杂豆的适用性，探索了不同杂豆粉的货架期。采用Plackett-Burman设计从8个影响绿豆粉发酵的因素中筛选出浸泡温度、均质压力、进口温度3个显著因素。采用Box-Behnken设计确定最佳工艺参数为浸泡温度50 ℃，均质压力200 Bar和进口温度115 ℃。该条件下绿豆粉中发酵乳杆菌活力的试验值为2.39×108 CFU/g，与理论值2.46×108 CFU/g相比，两者相差不显著。另外，通过考察该条件对红豆、鹰嘴豆、豇豆、豌豆和虎皮芸豆的影响，发现杂豆粉中的益生菌活性可以超过107 CFU/g，达到益生菌食品要求。其中，虎皮芸豆粉含量最高，达到3.27×108 CFU/g，表明绿豆的优化条件对这些杂豆具有普遍适用性。Arrhenius模型预测该系列杂豆粉产品贮藏1年的温度应低于10 ℃。杂豆粉中水分含量较低，有利于贮藏。

Optimization of Processing Conditions and Prediction of Shelf Life of Probiotic-rich Miscellaneous Bean Powder

Mung beans as raw materials were used to prepare probiotic-rich mung bean powder, the preparation process conditions were optimized. The applicability of the preparation process conditions to different miscellaneous beans were verified, and the shelf life of different miscellaneous bean powders were explored. The Plackett-Burman design was used to select soaking temperature, homogenization pressure, and inlet temperature as three of the eight significant factors that affect the fermentation in mung bean powder. The best process parameters were as follows: soaking temperature 50 ℃, homogenizing pressure 200 Bar and inlet temperature 115 ℃ as determined by the Box-Behnken design. Under these conditions, the experimental value of Lactobacillus fermentum in mung bean powder was 2.39×108 CFU/g, which was not significantly different from the predicted value of 2.46×108 CFU/g. In addition, by examining the effects of this optimized condition on red beans, chickpeas, cowpeas, peas and tiger skin kidney beans, it was found that the bacterial activity in the bean powder could exceed 107 CFU/g. This was met the requirements of probiotic foods. Among them, tiger skin kidney bean powder was the highest, reaching 3.27×108 CFU/g, indicating that these conditions had universal applicability for miscellaneous beans. The Arrhenius model predicted that the storage temperature of the bean powder products for one year should be less than 10 ℃. In addition, the moisture content in the miscellaneous bean powder were low, which were conducive to storage.