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| pH值对运动发酵单胞菌葡萄糖代谢酶活性影响的研究 | |
| 引用本文： | 陈玲,王成涛,孙宝国,曹雁平,童军茂.pH值对运动发酵单胞菌葡萄糖代谢酶活性影响的研究[J].中国酿造,2010(2). |
| 作者姓名： | 陈玲 王成涛 孙宝国 曹雁平 童军茂 |
| 作者单位： | 1. 北京工商大学,化学与环境工程学院,北京,100048;石河子大学,食品学院,新疆,石河子,832003 2. 北京工商大学,化学与环境工程学院,北京,100048 3. 石河子大学,食品学院,新疆,石河子,832003 |
| 基金项目： | 国家"973"计划项目，北京市属高校"中青年骨干人才培养计划"资助 |
| 摘 要： | 以运动发酵单胞菌(Zymomonas mobilis)ATCC31821为模式菌株,研究pH值条件对其葡萄糖代谢关键酶活力的影响.结果表明:发酵液pH5.5时,胞内乙醇脱氢酶、丙酮酸脱羧酶、葡萄糖激酶、葡萄糖-6-磷酸脱氢酶的活力较高,而异柠檬酸脱氢酶活性较低,能促进乙醇的生成;pH 5.0时,苹果酸脱氧酶的酶活力较低,使糖酵解反应向另一个方向发生偏移,促进乙醇的形成.pH 4.0～6.5时,丙酮酸激酶和甘油醛-3-磷酸脱氢酶的酶活力水平相差不大,说明pH值对这2种酶的活性影响甚微.因此,pH5.0～5.5,代谢途径(如糖酵解途径、ED途径等)中的胞内代谢酶活性增强,有利于乙醇的产生. |
| 关 键 词： | 运动发酵单胞菌 糖代谢 酶活力 代谢流量 |
Effects of pH on the enzyme activties in glucose metalolism of Zymomonas mobilis |
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| CHEN Ling,WANG Chengtao,SUN Baoguo,CAO Yanping,TONG Junmao.Effects of pH on the enzyme activties in glucose metalolism of Zymomonas mobilis[J].China Brewing,2010(2). | |
| Authors: | CHEN Ling WANG Chengtao SUN Baoguo CAO Yanping TONG Junmao |
| Affiliation: | CHEN Ling1,2,WANG Chengtao1*,SUN Baoguo1,CAO Yanping1,TONG Junmao2 (1.College of Chemistry , Environment Engineering,Beijing Technology , Business University,Beijing 100048,China,2.College of Food,Shihezi University,Shihezi 832003,China) |
| Abstract: | Zymomonas mobilis strain ATCC31821 is used to study the eff ect of different pH conditions on the enzyme activity in the glucose metalolism.The results showed that the activities of alcohol dehydrogenase(ADH),pyruvate decarboxylase(PDC),glucosekinase(GK)and glucose-6- phosphate dehydrogenase were high at pH 5.5,and the activity of isocitrate dehydrogenase(ICDH)was low at pH 5.5,which resulted in more carbon flux flow to ethanol.The activity of malate dehydrogenase(MDH)was lower at pH 5.0,which drive the gly... |
| Keywords: | Z mobilis glucose metabolism enzyme activity metabolic flux analysis(MFA) |
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