| 海茸中L-岩藻糖及硫酸基的测定 |
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| 引用本文： | 颜丽,刘秀河,赵方铭,李同乐.海茸中L-岩藻糖及硫酸基的测定[J].中国调味品,2020(2):137-141. |
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| 作者姓名： | 颜丽 刘秀河 赵方铭 李同乐 |
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| 作者单位： | 齐鲁工业大学（山东省科学院）;济南褐藻生物工程有限公司 |
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| 基金项目： | 十三五国家重点研发计划(2017YFB038600);国家级大学生创新创业训练计划项目(201710431046) |
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| 摘 要： | 采用比色法测定L-岩藻糖,L-岩藻糖的吸光值在0~18μg/mL呈良好的线性关系;采用热水浸提法提取海茸中L-岩藻糖,其含量为5.790%,回收率为94.46%,重复性和精密度良好。采用硫酸钡比浊法测定海茸中L-岩藻糖中硫酸基含量,硫酸根浓度的吸光值在0~24μg/mL范围内线性关系良好,测定SO42-含量为3.746%,回收率为95.28%,相对标准偏差为3.68%,重复性和精密度良好。最后测定海茸中褐藻胶含量为74.5%。
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| 关 键 词： | 海茸 L-岩藻糖 硫酸基含量 比色法 硫酸钡比浊法 |
Determination of L-Fucose and Sulfate in Seaweed |
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| YAN Li,LIU Xiu-he,ZHAO Fang-ming,LI Tong-le.Determination of L-Fucose and Sulfate in Seaweed[J].China Condiment,2020(2):137-141. |
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| Authors: | YAN Li LIU Xiu-he ZHAO Fang-ming LI Tong-le |
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| Affiliation: | (Qilu University of Technology(Shandong Academy of Sciences),Ji'nan 250353,China;Ji'nan Brown Algae Bioengineering Co.,Ltd.,Ji'nan 250353,China) |
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| Abstract: | L-fucose is determined by colorimetry,and the absorbance value of L-fucose has a good linear relationship from 0μg/mL to 18μg/mL,L-fucose is extracted by hot water extraction,the content is 5.790%,the recovery rate is 94.46%,the repeatability and precision are good.Barium sulfate turbidimetric method is used to determine the content of sulfuric acid in L-fucose of seaweed.The absorbance value of sulfuric acid has a good linear relationship in the range of 0~24μg/mL.The content of SO42- is 3.746%,the recovery rate is 95.28%,the relative standard deviation is 3.68%,and the repeatability and precision are good.Finally,the content of sodium alginate in seaweed is 74.5%. |
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| Keywords: | seaweed L-fucose sulfate content colorimetric method barium sulfate turbidimetry |
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