| 产葡萄糖氧化酶菌株的筛选及发酵培养基的优化 |
| |
| 引用本文： | 韩建春,冯镇,张宏伟.产葡萄糖氧化酶菌株的筛选及发酵培养基的优化[J].食品科学,2011(1). |
| |
| 作者姓名： | 韩建春 冯镇 张宏伟 |
| |
| 作者单位： | 东北农业大学食品学院; |
| |
| 摘 要： | 从土壤中筛选出了一株产葡萄糖氧化酶较高的菌株,利用响应面法对该菌株的产酶培养基进行优化以提高产酶量。响应面法优化的发酵培养基组成为:葡萄糖109.41g/L、复合氮源(m(月示蛋白胨):m((NH4)2SO4)=3:1)37.36g/L、吐温-80 37.15g/L、KH2PO4 2g/L、MgSO4.7H2O 0.7g/L和KCl 0.5g/L。采用该优化培养基所得葡萄糖氧化酶的活力为1.54U/mL,较优化前提高了31.6%。
|
| 关 键 词： | 葡萄糖氧化酶 酶活力 培养基优化 响应面 |
Strain Screening and Optimization of Fermentation Medium for Higher Production of Glucose Oxidase |
| |
| HAN Jian-chun,FENG Zhen,ZHANG Hong-wei.Strain Screening and Optimization of Fermentation Medium for Higher Production of Glucose Oxidase[J].Food Science,2011(1). |
| |
| Authors: | HAN Jian-chun FENG Zhen ZHANG Hong-wei |
| |
| Affiliation: | HAN Jian-chun,FENG Zhen,ZHANG Hong-wei(College of Food Science,Northeast Agricultural University,Harbin 150030,China) |
| |
| Abstract: | Glucose oxidase(GOD) has gained considerable commercial importance due to its multitude of applications in the food,pharmaceutical,biotechnology and other industries.In the present investigation,a high GOC-producing strain was isolated from the soil of Wenwu Xuefeng farm in Fuzhou,and the fermentation medium was optimized by response surface methodology for higher GOD production by the isolated strain.The optimal medium composition determined consisted of glucose 109.41 g/L,complex nitrogen source(proteose ... |
| |
| Keywords: | glucose oxidase enzyme activity medium optimization response surface methodology |
| 本文献已被 CNKI 等数据库收录！ |
|
