菊芋酶解制备低聚果糖糖浆的工艺优化及功能评价

菊芋块茎富含菊糖，是制备低聚果糖（Fructooligosaccharides,FOS）的主要原料之一，新鲜菊芋块茎直接酶法加工用于功能性糖浆的制备可以丰富菊芋综合加工的应用。本研究以新鲜菊芋块茎为原料，通过系统研究菊芋内源酶、菊粉内切酶、葡聚糖内切酶、木聚糖酶、聚半乳糖醛酸酶和单宁酶在鲜菊芋酶法加工制备低聚果糖糖浆中的作用规律和酶解效果，建立并优化了酶法制备低聚果糖糖浆的工艺。结果表明，最优酶解工艺如下：菊芋浆在50℃和pH5.0条件下，加入0.08 U/g单宁酶酶解4 h，再加入0.08 U/g葡聚糖内切酶、0.08 U/g木聚糖酶、0.07 U/g聚半乳糖醛酸酶和12 U/g菊糖内切酶组合酶解8 h，酶解液浓缩2倍后获得低聚果糖糖浆成品。成品中低聚果糖和单宁的含量分别为53.72和3.11 g/L,DPPH自由基清除率、羟基自由基清除率和总抗氧化能力分别为82.23%、30.47%和2.78μmol/mL。以制备的低聚果糖糖浆为唯一碳源替代MRS培养基中的葡萄糖，植物乳杆菌、嗜热链球菌和副干酪乳杆菌的生长速率较未经酶解的菊芋原浆作为MRS培养基的唯一碳源时，分别提高了33.33%...

Optimization of Enzymatic Hydrolysis of Jerusalem Artichoke to Produce Fructooligosaccharide Syrup and Its Function Evaluation

Jerusalem artichoke tuber (JAT) is rich in inulin and it is considered as one of the most important feedstocks for fructooligosaccharides (FOS) production. Direct enzymatic production of functional syrup from fresh JAT can benefit comprehensive processing of Jerusalem artichoke. In this study, the effects of various enzymes (endogenous hydrolases, endoinulinase, endoglucanase, xylanase, polygalacturonase and tannase) on fructooligosaccharide production from JAT were systematically studied and enzymatic process were further optimized. The optimal process was: first loading tannase (0.08 U/g) at 50 °C and pH 5 for 4 h, and then loading endoglucanase (0.1 U/g), xylanase (0.08 U/g), polygalacturonase (0.07 U/g) and endoinulinase (12 U/g) for 8 h. The FOS syrup was obtained by concentrated reaction solution for tow folds. The FOS and total polyphenols in FOS syrup reached 53.72 g/L and 3.11 g/L. The DPPH free radical scavenging rate, hydroxyl radical scavenging rate and total antioxidant capacity of FOS syrup were 82.23%, 30.47% and 2.78 μmol/mL, respectively. Replacing glucose in MRS medium with prepared fructooligosaccharide syrup as sole carbon source, the growth rates of Lactobacillus plantarum, Streptococcus thermophilus and Lactobacillus paracasei increased by 33.33%, 60.47% and 148.15%, respectively, compared with the unenzymatically hydrolyzed Jerusalem artichoke puree as the sole carbon source of MRS medium. The main results of this study provide a theoretical basis for the enzymatic production of prebiotic food from Jerusalem artichoke.