响应面法优化茴香精油提取工艺及其微乳特性和货架期研究

本研究通过响应面法对茴香精油提取工艺进行优化，然后以茴香精油为油相、Tween 80+40为表面活性剂、丙三醇为助表面活性剂制备茴香精油微乳液体系，利用流变法、粒度仪及还原反应，对茴香精油微乳构型、粒径、总还原力及贮藏性进行探究。结果表明，当料液比1:8.40 g/mL，浸提时间4.09 h，颗粒粒度80目，浸提温度80 ℃时，茴香精油得率高达15.99%±0.10%。制备的茴香精油微乳粒径为30.92±0.13 nm，当水分含量高于60%时，为稳定的O/W型微乳。茴香精油的乳化明显提升了茴香精油的总还原力。根据化学反应动力学原理，建立Arrhenius方程为:k=2518.85·e?35583.09/RT，该模型预测茴香精油微乳在20、35、50和65 ℃下能贮藏 1899.18、932.03、492.94和 271.51 h，最大相对误差8.73%，误差较小，可准确预测该微乳的货架期。

Optimization of Extraction Process of Fennel Essential Oil by Response Surface Methodology and Its Microemulsion Characteristics and Shelf Life

In this study, the extraction process of fennel essential oil was optimized by response surface methodology. Fennel essential oil was used as oil phase, Tween 80+40 as surfactant and glycerol as cosurfactant to prepare fennel essential oil microemulsion system. The structure, particle size, total reducing power and storage property of fennel essential oil microemulsion were investigated by rheology, particle size analyzer and reduction reaction. The results showed that the yield of essential oil of fennel was as high as 15.99%±0.10% when the ratio of material to liquid was 1:8.40 g/mL, the extraction time was 4.09 h, the particle size was 80 mesh, and the extraction temperature 80 ℃. The diameter of fennel essential oil microemulsion was 30.92±0.13 nm, and when the moisture content was higher than 60%, it was a stable O/W microemulsion. The emulsification of fennel essential oil significantly improved the total reducing power of fennel essential oil. According to the principle of chemical reaction kinetics, the Arrhenius equation was established as k=2518.85·e?35583.09/RT. The model predicted that the fennel essential oil microemulsion could store 1899.18, 932.03, 492.94 and 271.51 h at 20, 35, 50 and 65 ℃, the maximum relative error was 8.73%, and the error was small, which could accurately predict the shelf life of the microemulsion.