黄酒对小鼠抗疲劳能力和衰老小鼠免疫器官的影响

目的：研究黄酒对正常小鼠抗疲劳能力和D-半乳糖所致亚急性衰老小鼠免疫器官的影响。方法：抗疲劳能力实验中，小鼠分别灌胃给予双蒸水（0.25 mL/d）、高（0.4 mL/d）、中（0.25 mL/d）、低（0.15 mL/d）剂量的黄酒（乙醇体积分数为8%）15 d，小鼠游泳后检测肝糖原（hepatic glycogen，HG）、尿素氮（blood ureanitrogen，BUN）和血乳酸（blood lactic acid，BLA）含量的变化。在黄酒对亚急性衰老小鼠免疫器官影响的实验中，采用腹腔注射D-半乳糖建立亚急性衰老小鼠模型，分别灌胃双蒸水、酒精和不同剂量的黄酒，每天1 次，连续灌胃56 d，测定小鼠胸腺指数、脾脏指数和亚急性衰老模型建立前后的体质量增值。结果：黄酒能显著提高小鼠体内HG的含量（P＜0.01）；增加运动后小鼠BLA增长率（P＜0.05）和BUN的廓清率（P＜0.05）；黄酒能抑制衰老小鼠胸腺（P＜0.01）和脾脏（P＜0.05）质量的减少，有助于衰老小鼠的体质量增长（P＜0.05）。结论：黄酒能提高小鼠的抗疲劳能力，增强衰老小鼠的免疫能力。

Anti-Fatigue Effect of Chinese Rice Wine in Normal Mice and Its Influence on Immune Organ Indexes of Aging Mice

Objective: The aim of this study was to evaluate the anti-fatigue effect of Chinese rice wine (CRW) in normal
mice and its influence on immune organ indexes of D-galactose-induced aging mice. Methods: Mice were randomly divided
into four groups: control, low-dose CRW, middle-dose CRW and high-dose CRW groups, which were given by gavage
0.25 mL of distilled water, and 0.15, 0.25 and 0.4 mL of CRW for 15 consecutive days, respectively for exploring the antifatigue
effect of CRW. Then, changes in hepatic glycogen, blood urea nitrogen (BUN) and blood lactic acid (BLA) contents
in mice after forced swimming were measured. A mouse model of sub-acute aging was established by intraperitoneal
injection of D-galactose and the aging mice were respectively given distilled water, aqueous ethanol and CRW once a day
by intragastric administration for 56 days. Finally, the mice were sacrificed, for the measurement of thymus index, spleen
index and body weight gain. Results: CRW could significantly increase hepatic glycogen level (P < 0.01) and enhance postexercise
increase in BLA level (P < 0.05) and the clearance rate of BUN level (P < 0.05). In addition, CRW significantly
inhibited the decrease in thymus (P < 0.01) and spleen (P < 0.05) weights and increased body weight (P < 0.05). Conclusion:
CRW can exert an anti-fatigue effect and enhance immune function in aging mice.