温州市食品中沙门菌污染状况及特征分析

目的了解温州市食品中沙门菌的污染状况,分析分离的沙门菌血清型分布、耐药性及脉冲场凝胶电泳(PFGE)分子分型特征。方法依据GB 4789.4—2016《食品安全国家标准食品微生物学检验沙门氏菌检验》进行菌株分离鉴定及血清学分型,采用微量肉汤稀释法进行药敏试验,PFGE法进行分子分型。结果 6类食品2 039份样品中,37份样品检出沙门菌,检出率为1.8%,其中生禽肉和生畜肉检出率较高,分别为6.9%(20/290)和3.4%(10/290)。37株沙门菌分属16种血清型,居前三位分别为鼠伤寒沙门菌、德尔卑沙门菌和肠炎沙门菌。81.1%(30/37)的菌株对17种抗生素产生不同程度的耐药,呈现24种耐药谱,多重耐药率为56.8%(21/37)。PFGE图谱分为31种PFGE带型,呈多态性。结论沙门菌在温州市食品中存在一定的污染率,耐药情况形式严峻,PFGE图谱的聚集性与沙门菌的血清型有一定的联系,与耐药谱之间的关联性并不明确。

Contamination and characteristics of foodborne Salmonella in Wenzhou

Objective To investigate Salmonella contamination in food in Wenzhou, and to analyze the serotype distribution, drug resistance, and pulsed-field gel electophroresis(PFGE)patterns. Methods According to GB 4789.4-2016, the Salmonella strains were isolated, identified and serotyped. The broth microdilution method was used for drug sensitivity test. All strains were subtyped by PFGE. Results Six categories of 2 039 food samples were collected. 37 Salmonella strains were isolated with a positive isolation rate of 1.8%. Raw poultry meat and raw livestock meat got the highest detection rates with 6.9%(20/290) and 3.4%(10/290), respectively. The 37 Salmonella isolates belonged to 16 serotypes,including S. Typhimurium, S. Derby and S. Enteritidis as the dominant serotypes. 81.1%(30/37) of the strains showed different resistance levels to 17 kinds of antibiotics, and displayed 24 kinds of multiple drug resistant spectrums. The multiple antibiotic resistance rate reached 56.8%(21/37). The 37 isolates were divided into 31 PFGE patterns which showed polymorphism. Conclusion Food was contaminated to some degree by Salmonella in Wenzhou, and the antibiotic resistance was severe. The aggregation of PFGE patterns was related to the serotype of Salmonella, but had no obvious relation to the resistant spectrum.