高效液相色谱法测定低聚半乳糖的含量

研究了高效液相色谱法测定乳制品及糖浆中低聚半乳糖含量的方法。以水和乙腈为流动相,用氨基柱将葡萄糖,半乳糖和乳糖与其他物质分离,用示差折光检测器检测,定量。通过计算低聚半乳糖酶解成的半乳糖含量,来对低聚半乳糖定量。葡萄糖,半乳糖,乳糖和低聚半乳糖的相对标准偏差分别为2.72%、4.16%、1.16%、4.26%。此法的线性相关系数为0.9990～0.9995,回收率达到95%～110%。此法能快速准确的测定低聚半乳糖的含量。

Determination of galactooligosaccharide by high performance liquid chromatography

HPLC method was established to determine the galactooligosaccharide in dairy products and syrup.Glucose,galactose and lactose were simultaneously assayed on a NH 2 column by using a refractive index detector and a mobile phase of acetonitrile-water.β-Galactosidase can hydrolyze GOS into galactose.Then GOS are calculated from concentrations of lactose and galactose.The relative standard deviation was 2.72%,4.16%,1.16%,4.26% for glucose,galactose,lactose and galactooligosaccharide respectively.The method presented linear correlation coefficient between 0.9990 and 0.9995.The recovery ranged from 95% to 110%.This method was proved to be accurate and rapid.