柠檬桉叶的HS-SDME-GC/MS谱图及其主成分分析

采用顶空单液滴微萃取-气相色谱/质谱法(HS-SDME-GC/MS)测定了13个不同产地的柠檬桉叶并结合主成分分析法建立判别模型,采用正交法优化其实验条件。结果表明,单液滴微萃取优化条件为:10 mL顶空瓶中1.0 g样品、60℃顶空40 min、苯甲酸乙酯吸附进而吸收柠檬桉叶中的化学物质,比对NIST 14谱库并结合相关文献鉴定柠檬桉叶中30种化学成分,化学成分按照烯类、醇类、酯类和醛类进行分类,并建立主成分分析判别模型。方法简单、快速、准确,对天然产物进行有效的产地溯源,实用性良好。

HS-SDME-GC/MS Chromatogram and Principal Component Analysis for Eucalyptus Citriodora Hook.f.Leaves

The samples of Eucalyptus citriodora Hook.f.leaves from thirteen different regions were analyzed by headspace-single drop microextraction-gas chromatography/mass spectrometry(HS-SDME-GC/MS).Then principal component analysis(PCA) discriminant model was established.At the same time,the experimental conditions were optimized via orthogonal method.An amount of 1.0 g raw powder in 10 mL headspace vial was heated up to 60 ℃ for 40 min,meanwhile the compounds of Eucalyptus citriodora Hook.f.leaves were volatilized and then adsorbed by the single drop of ethyl benzoate.Mass spectroscopy was applied to characterize 30 components in samples by referring to the NIST 14 mass spectrometry database and related literatures.In summary,HS-SDME-GC/MS with PCA can be used to identify regions and has great practicability.Thus,the results proved HS-SDME-GC/MS is a simple,rapid and accurate method.