不同缓冲溶液对BSA蛋白硝化和脂质过氧化的影响

研究了2种不同形态铁（EDTA-Fe(III)和柠檬酸铁）在5种常用缓冲溶液（碳酸盐缓冲液、Tris-HCl缓冲液、磷酸盐缓冲液、柠檬酸和Hepes缓冲液）中催化H₂O₂-NO₂?导致牛血清白蛋白（BSA）发生的蛋白硝化和脂质过氧化，以此来探讨缓冲溶液及催化剂的选择对蛋白质硝化和脂质过氧化的影响。实验表明，在5种缓冲溶液中，EDTA-Fe(III)和柠檬酸铁均能够有效地催化H₂O₂-NO₂?，导致BSA发生蛋白质硝化和脂质过氧化。其中，蛋白质硝化在碳酸盐缓冲溶液中最高，而脂质过氧化在Tris-HCl缓冲液中最高。不同缓冲液中，蛋白质硝化和脂质过氧化的程度有所不同，这表明缓冲溶液对蛋白质硝化和脂质过氧化有一定程度的影响。对于同一种缓冲溶液而言，EDTA-Fe(III)和柠檬酸铁催化H₂O₂-NO₂?导致BSA发生的蛋白质硝化和脂质过氧化也有所差异，表明催化剂的选择同样对蛋白质硝化和脂质过氧化存在一定的影响。

Effects of Different Buffers on Protein Nitration and Lipid Peroxidation of BSA

This article studies two different forms of iron (EDTA- Fe (III) and ferric citrate) in five kinds of common buffer solution (Na₂CO₃ buffer, Tris-HCl buffer, PBS buffer, citric acid buffer and Hepes buffer) catalysis H₂O₂-NO₂? to cause protein nitration and lipid peroxidation of bovine serum albumin (BSA), so as to explore the influence of buffer solution and the choice of catalysts on protein nitration and lipid peroxidation. The results show that EDTA-Fe(III) and ferric citrate can effectively catalyze H₂O₂-NO₂? to lead protein nitration and lipid peroxidation of BSA in all the five buffer solutions. Protein nitration was the highest in Na₂CO₃ buffer, while lipid peroxidation was the highest in Tris-HCl buffer. In different buffer, the degree of protein nitration and lipid peroxidation was different, which indicated that the buffer has an effect on protein nitration and lipid peroxidation. In the same buffer solution, protein nitration and lipid peroxidation of BSA caused by EDTA-Fe(III)-H₂O₂-NO₂? were different with by ferric citrate -H₂O₂-NO₂?. It shows that the choice of catalysts also influent the protein nitration and lipid peroxidation.