一株具降胆固醇功能的海洋源植物乳杆菌的筛选及其益生性能分析

本文采用邻苯二甲醛法从喙鲸内脏中筛选分离出一株高效降胆固醇能力的乳酸菌HJ-S2,考察并研究了乳酸菌HJ-S2的体外益生性能。通过耐酸、耐胆盐测定菌株的益生作用,研究了其对致病菌的抑菌作用及对人结肠癌细胞HT-29的粘附能力,利用形态学、API50CH和16S rDNA对菌株进行鉴定。乳酸菌HJ-S2体外降胆固醇能力达到48.82%,在p H值3的酸性环境中培养3h耐受率达到82.73%,在含0.3%的胆盐环境中培养3 h耐受率达80.62%。菌株代谢物对致病菌大肠杆菌、金黄色葡萄球菌和沙门氏菌均有一定的抑菌作用,且菌株对人结肠癌细胞HT-29很强的粘附能力,粘附细菌数为132.52,经鉴定为植物乳杆菌Lactobacillus plantarum,并命名为HJ-S2(CGMCCNO:17720)。筛选得到的植物乳杆菌HJ-S2体外具有高效降胆固醇能力,可进一步开发为功能性微生态制剂。

Screening of a Marine Lactobacillus plantarum with Cholesterol-lowering Effect

In this work, a strain of lactic acid bacteria HJ-S2 with high effective cholesterol-lowering was isolated from Beak Whale viscera using o-benzaldehyde method, and the probiotic performance of Lactobacillus plantarum HJ-S2 were investigated in vitro. Acid-resistant and cholestate-tolerant were carried out to detect the probiotic performance of the strain, and antibacterial effect on pathogenic bacteria and adhesion ability to human colon cancer cell HT-29 were also studied. Morphology, API50CH and 16S rDNA were used to identify the strain. The results showed thatthe cholesterol-lowering rate of this lactic acid bacteria HJ-S2 was 48.82% in vitro. Under being cultured for 3 h at pH 3 or in solvent containing 0.3% bile salt, the tolerance rate reached 82.73% and 80.62% respectively. The result of antibacterial analysis suggested metabolites of the strain HJ-S2 have certain inhibitory effect on E.coli, S.areaus and Salmonella. Analysis of adhesion to human colon cancer cell HT-29 of 132.52. The strain was identified as Lactobacillus plantarum and labled as HJ-S2 (CGMCC NO: 17720). The Lactobacillus plantarum HJ-S2 has effective high cholesterol-lowering and can provide a reference for further mircro-ecologicol agent development.