醇化方式对津巴布韦烟叶微生物和香味成分的影响

为了解醇化方式对烟叶微生物和烟叶香味成分的影响，采用MiSeq高通量测序技术和气相色谱-质谱法，分析了不同醇化库（保湿库、机械库和自然库）不同醇化时间（3、9、12、15和18个月）津巴布韦烟叶微生物多样性及香味成分的差异。结果表明：(1)同一醇化库内醇化时间为3、9和12个月时物种组成相似度较高，但不同醇化库之间的物种组成差异较大；(2)在门、属水平上，醇化时间和醇化库对菌落的相对丰度有一定影响，醇化18个月后，机械库中烟叶变形菌门（Proteobacteria）、放线菌门（Actinobacteria）和厚壁菌门（Firmicutes）的总相对丰度达最大值，假单胞菌属（Pseudomonas）、泛菌属（Pantoea）、鞘氨醇单胞菌属（Sphingomonas）、节杆菌属（Arthrobacter）和甲基杆菌属（Methylobacterium）的总相对丰度达最大值；(3)功能预测表明，在一级功能层共有7类代谢通路，参与代谢的细菌占绝对优势；(4)在二级功能层共有41种代谢通路，参与膜运输、能量代谢和碳水化合物代谢等细菌的相对丰度较大。醇化18个月后，机械库中参与碳水化合物代谢、氨...

Effects of aging methods on microorganisms and aroma components of Zimbabwe tobacco leaves

In order to investigate the effects of aging methods on microorganisms and aroma components in tobacco leaves, the differences in microbial diversity and aroma components of Zimbabwe tobacco leaves aged for different durations (3, 9, 12, 15 and 18 months) in different aging warehouses (moisturizing warehouse, mechanical warehouse and natural warehouse) were analyzed by MiSeq high-throughput sequencing technology and gas chromatography-mass spectrometry. The results showed that: 1) The species composition similarity was higher among the leaves aged for 3, 9, and 12 months in the same aging warehouse, but the species composition of the leaves in different aging warehouses differed greatly. 2) At phylum and genus levels, both aging duration and aging warehouse had certain influences on the relative abundance of colonies. After aging for 18 months in the mechanical warehouse, the total abundance of Proteobacteria, Actinobacteria and Firmicutes and the total relative abundance of Pseudomonas, Pantoea, Sphingomonas, Arthrobacter and Methylobacterium reached their maxima. 3) The functional prediction indicated that there were 7 types of metabolic pathways in the primary functional layer, and the bacteria involved in metabolism were dominant. 4) There were 41 metabolic pathways in the secondary functional layer, the relative abundance of bacteria involved in membrane transport, energy metabolism and carbohydrate metabolism was higher. After aging for 18 months in the mechanical warehouse, the bacteria involved in carbohydrate metabolism, amino acid metabolism and lipid metabolism had higher relative abundance. 5) With the proceeding of aging treatments, the contents of alcohols and ketones arom components, neophytadiene and the total contents of aroma components in tobacco leaves increased gradually, and the total contents of aroma components in tobacco leaves aged in the mechanical warehouse were higher than those in the moisturizing warehouse and natural warehouse.