| 基质辅助激光解吸电离飞行时间质谱定性分析啤酒中糖类条件的优化 |
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| 引用本文： | 孟贤仁,谢明勇,聂少平,王君巧.基质辅助激光解吸电离飞行时间质谱定性分析啤酒中糖类条件的优化[J].食品与机械,2020(4):86-90. |
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| 作者姓名： | 孟贤仁 谢明勇 聂少平 王君巧 |
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| 作者单位： | 南昌大学食品科学与技术国家重点实验室,江西 南昌 330047 |
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| 基金项目： | 国家重点研发课题(编号:2017YFC1600405)；江西省重大研发专项(编号:20165ABC28004)；国家自然科学基金青年科学基金项目(编号:31801495) |
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| 摘 要： | 通过选择不同基质(2,5-二羟基苯甲酸、super DHB、2,4,6-三羟基苯乙酮)和设置不同啤酒样品稀释倍数(从原液到1 000倍稀释),优化了MALDI TOF MS定性分析啤酒中糖类的试验条件。研究结果显示,3种基质中,2,4,6-三羟基苯乙酮应用于MALDI TOF MS对啤酒中糖类表征的能力优于另外两种基质。当以2,4,6-三羟基苯乙酮为基质,啤酒样品稀释10倍时,MALDI TOF MS可达良好的定性分析效果。
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| 关 键 词： | 基质辅助激光解吸电离飞行时间质谱 啤酒 糖类 |
Optimization on qualitative analysis of saccharides in beer by MALDI-TOF MS |
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| MENG Xian-ren,XIE Ming-yong,NIE Shao-ping,WANG jun-qiao.Optimization on qualitative analysis of saccharides in beer by MALDI-TOF MS[J].Food and Machinery,2020(4):86-90. |
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| Authors: | MENG Xian-ren XIE Ming-yong NIE Shao-ping WANG jun-qiao |
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| Affiliation: | State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, Jiangxi 330047 , China |
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| Abstract: | In this study, the experimental condition for qualitative analysis of saccharides in beer was optimized by choosing different matrices, including. 2,5-dihydroxybenzoic acid (2,5-DHB), super DHB, 2,4,6-trihydroxyacetophenone, and setting different beer concentrations from undiluted to 1 000-fold dilution. Results indicated that 2,4,6-trihydroxyacetophenone was better than 2,5-dihydroxybenzoic acid and super DHB in the application of characterizing saccharides in beer by MALDI TOF MS. When the beer sample is diluted 10 times with 2,4,6-trihydroxyacetophenone as matrix, MALDI TOF MS performed well in qualitative analysis of saccharides in beer. |
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