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| 霍山蹦蝗中黄酮类化合物提取工艺与检测方法研究 | |
| 引用本文： | 李熊利,钟玉林,袁丹妮,徐兰英,阮芳,黄文.霍山蹦蝗中黄酮类化合物提取工艺与检测方法研究[J].食品与机械,2012,28(2):131-134,173. |
| 作者姓名： | 李熊利 钟玉林 袁丹妮 徐兰英 阮芳 黄文 |
| 作者单位： | 1. 华中农业大学食品科学技术学院,湖北武汉430070;经济林木种质改良与林产品综合利用湖北省重点实验室,湖北黄冈438000 2. 经济林木种质改良与林产品综合利用湖北省重点实验室,湖北黄冈,438000 3. 华中农业大学食品科学技术学院,湖北武汉,430070 |
| 基金项目： | 湖北省自然科学基金，湖北省教育厅自然科学重大项目，黄冈市科技三项经费计划 |
| 摘 要： | 考察不同因素对霍山蹦蝗中黄酮类化合物提取的影响。通过正交试验及SPSS分析,得出其优化工艺条件为温度85℃、甲醇浓度80%、时间2h、料液比1∶50(m∶V),提取率为2.02%。运用铝盐显色法和高效液相色谱法分别测出霍山蹦蝗中总黄酮含量为20.198,4.047mg/g(干重),结果表明铝盐显色法可能受杂质酚类干扰,而高效液相色谱法能很好的将不同黄酮类化合物与杂质分离开,因此检测结果更准确。 |
| 关 键 词： | 蹦蝗 黄酮 提取 铝盐显色分光光度 高效液相色谱 |
Study on extraction process and detection method of flavonoids from Sinopodisma houshana |
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| LI Xiong-li , ZHONG Yu-lin , YUAN Dan-ni , XU Lan-ying , RUAN Fang , HUANG Wen.Study on extraction process and detection method of flavonoids from Sinopodisma houshana[J].Food and Machinery,2012,28(2):131-134,173. | |
| Authors: | LI Xiong-li ZHONG Yu-lin YUAN Dan-ni XU Lan-ying RUAN Fang HUANG Wen |
| Affiliation: | 1(1.College of Food Science and Technology,Huazhong Agricultural University,Wuhan,Hubei 430070,China;2.Hubei Key Laboratory of Economic Forest Germplasm Improvement and Resources Comprehensive Utilization,Huanggang,Hubei 438000,China) |
| Abstract: | Orthogonal experiment and SPSS analysis were used to investigate the different influence factors of the flavonoids extraction from Sinopodisma houshana Huang.The results showed that a optimum condition of the flavonoids extraction from Sinopodisma houshana was got when solid-liquid ratio equals to 1∶50 with the 80% methanol,and then extracting for 2 h under the extraction temperature of 85 ℃,and the extraction ratio was 2.02%.The total flavonoids content of Sinopodisma houshana were 20.198 mg/g and 4.047 mg/g,respectively(dry weight) by means of aluminium salt-coloration spectrophotometry method and high performance liquid chromatography.The results showed that the high-performance liquid chromatography could separate different flavonoids from impurities in Sinopodisma houshana,while aluminium salt-coloration spectrophotometry method was interfered by other phenols in it.So the high-performance liquid chromatography was a better method to detect flavonoids. |
| Keywords: | Sinopodisma flavonoids extraction aluminium salt-coloration spectrophotometry HPLC |
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