红车轴草不同部位中异黄酮含量的测定

采用反相高效液相色谱法对红车轴草不同部位(花、茎和叶)中四种主要异黄酮(鸡豆黄素A、芒柄花黄素、染料木素和大豆黄素)的含量进行了测定,结果表明:以占干物质计,4种异黄酮总量,叶中含量最高(0.856%),茎次之(0.403%),花中含量较低(0.258%)。鸡豆黄素A和芒柄花黄素是红车轴草中两种最主要的异黄酮,主要分布在叶中,其次是茎,花中含量较低。染料木素和大豆黄素,无论是花、茎还是叶,含量均较低。

Determination of Isoflavones in Different Parts of Trifolium pratense by High Performance Liquid Chromatography

HPLC was used for separation and determination of four isoflavone compounds (biochanin A, formononetin,genistein, and daidzein) in different parts (leaf, flower, and stem) of Trifolium pratense. The results showed that there weredifferent distributions of the examined isoflavone compounds among different plant organs. The contents (dry weight) of totalfour isoflavone compounds in the leaf, stem and flower of Trifolium pratense were 0.856%, 0.403%, and 0.258%, respectively,among which the highest was in the leaf and the lowest in the flower. Biochanin A and formononetin were two kinds ofpredominant isoflavones in Trifolium pratense. The content (dry weight) of biochanin A in different parts was in a range from0.077% to 0.358%, among which the highest was in the leaf and the lowest in the stem. The content of formononetin in differentparts was in a range from 0.106% to 0.437%, among which the highest was in the leaf and the lowest in the flower. However, theamount of genistein and daidzein in different parts was low, whose content (dry weight) varied from 0.013% to 0.044%.