| 生物胺高效液相色谱法测定条件的选择与优化 |
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| 引用本文： | 翟红蕾,杨贤庆,郝淑贤,岑剑伟,魏涯,石红.生物胺高效液相色谱法测定条件的选择与优化[J].食品科学,2011,32(18):180. |
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| 作者姓名： | 翟红蕾 杨贤庆 郝淑贤 岑剑伟 魏涯 石红 |
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| 作者单位： | 中国水产科学研究院南海水产研究所;上海海洋大学食品学院; |
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| 基金项目： | 国家农业产业技术体系项目(nycytx-48); 国家公益性行业(农业)科研专项(201003055-06); 国家农业科技成果转化项目(2010GB23260577;2009GB2E200303;2010GB2E000335); “十一五”广东省科技计划项目(2009A020700004;2008A020100006;2009B020201003); 广东省海洋渔业科技推广项目(A200899B02A200901C01) |
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| 摘 要： | 利用反相高效液相色谱、荧光/二极管阵列检测器对8种生物胺进行测定,通过对流动相选择、流动相梯度洗脱设定、检测器比较、检测波长比较及其他条件的探讨,得出高效液相色谱法测定生物胺的最佳色谱条件。结果确定,采用乙腈-乙酸铵-超纯水作为流动相经梯度洗脱,8种生物胺在30min内全部被分离出且峰形对称无拖尾和前伸现象。改进后的色谱条件可节约样品分析时间,荧光检测器的高选择性和灵敏性能够满足高精确度生物胺检测分析的要求。
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| 关 键 词： | 高效液相色谱 生物胺 条件优化 |
Optimization of Operating Conditions for HPLC Determination of Biogenic Amines |
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| ZHAI Hong-lei,YANG Xian-qing,HAO Shu-xian,CEN Jian-wei,WEI Ya,SHI Hong.Optimization of Operating Conditions for HPLC Determination of Biogenic Amines[J].Food Science,2011,32(18):180. |
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| Authors: | ZHAI Hong-lei YANG Xian-qing HAO Shu-xian CEN Jian-wei WEI Ya SHI Hong |
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| Affiliation: | ZHAI Hong-lei1,2,YANG Xian-qing1,*,HAO Shu-xian1,CEN Jian-wei1,WEI Ya1,SHI Hong1 (1.South China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Guangzhou 510300,China,2.College of Food Science and Technology,Shanghai Ocean University,Shanghai 201306,China) |
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| Abstract: | In order to establish a reversed-phase HPLC method for the determination of 8 biogenic amines,mobile phase composition,elution mode,detector type and detection wavelength were optimized.Gradient elution using a mobile phase composed of acetonitrile,ammonium acetate and ultra-pure water proved optimal,and 8 biogenic amines were completely separated within 30 min,showing symmetrical peak shapes without tails and extension.The optimization resulted in a time-saving analytical method.Moreover,fluorescence detec... |
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| Keywords: | high performance liquid chromatography(HPLC) biogenic amines measurement conditions optimization |
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