| 沙丁胺醇人工抗原合成及鉴定研究 |
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| 引用本文： | 袁利鹏,孙远明,雷红涛,沈玉栋,黄晓钰,潘科,黄杰.沙丁胺醇人工抗原合成及鉴定研究[J].食品科学,2006,27(12):276-280. |
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| 作者姓名： | 袁利鹏 孙远明 雷红涛 沈玉栋 黄晓钰 潘科 黄杰 |
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| 作者单位： | 华南农业大学食品质量安全研究所 广东省教育厅食品质量安全重点实验室； |
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| 基金项目： | 国家自然科学基金项目（20377016）;广东省自然科学基金项目（032240）;广州市科技攻关项目（2004Z2-E0031） |
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| 摘 要： | 用琥珀酸酐将沙丁胺醇活化后制备出半抗原沙丁胺醇半琥珀酸,经电喷雾电离质谱(ESI-MS)、红外光谱(IR)和核磁共振(1H-NMR)鉴定合成成功,同时证实琥珀酸酐连结在沙丁胺醇乙醇胺的羟基处,且半抗原以内胺盐形式存在。用混合酸酐、活泼酯方法将沙丁胺醇琥珀半酸分别与牛血清白蛋白(BSA)和卵清白蛋白(OVA)偶联制备免疫抗原和包被抗原,经紫外吸收法鉴定偶联成功,偶联比分别为12.8:1、23:1。本研究为进一步制备沙丁胺醇抗体奠定了基础。
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| 关 键 词： | 沙丁胺醇 半抗原 鉴定 |
| 文章编号： | 1002-6630（2006）12-0276-05 |
| 收稿时间： | 2006-08-31 |
| 修稿时间： | 2006-08-31 |
Synthesis and Identification for Salbutamol Antificial Antigen |
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| YUAN Li-peng,SUN Yuan-ming,LEI Hong-tao,SHEN Yu-dong,HUANG Xiao-yu,PAN Ke,HUANG Jie.Synthesis and Identification for Salbutamol Antificial Antigen[J].Food Science,2006,27(12):276-280. |
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| Authors: | YUAN Li-peng SUN Yuan-ming LEI Hong-tao SHEN Yu-dong HUANG Xiao-yu PAN Ke HUANG Jie |
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| Affiliation: | Key Laboratory of Food Quality and Safety, Education Department of Guangdong Province, Research Institute of Food Quality and Safety, South China Agricultural University, Guangzhou 510642, China |
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| Abstract: | Salbutamol is actived by succinic anhydride and converted into salbutamol-succinate as the hapten. The results of IR, 1H-NMR and MS indicated that the hapten was synthesized successfully and identified as an inner salt. Salbutamol-succinate was linked to carrier protein bovine serum albumin (BSA) as immunogen, and ovalbumin(OVA) as coating antigen by mixed anhydride method and NHS ester method respectively. Antigens SAL-BSA and SAL-OVA were prepared successfully and confirmed by ultraviolet spectrum. The conjugation ratio of SAL-BSA and SAL-OVA were 12.8:1, 23:1, respectively. |
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| Keywords: | salbutamoh hapten identification |
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