高效液相色谱法分析北方部分地区蜂胶醇提物成分

研究北方部分地区的蜂胶提取物中组分含量的差异并对成分分析。采用乙醇水溶液对中国北方11个省市的蜂胶进行提取，以NaNO2-Al(NO3)3比色法和Folin-Ciocalteu试剂比色法分别测定蜂胶不同提取物的总黄酮和总酚酸含量；并且借助标准品以高效液相色谱法对不同提取物中的酚类化合物进行初步定性和定量，进而模拟蜂胶的区域性。结果表明：不同地区的蜂胶含有的总黄酮和总酚酸不同，其中咖啡酸、坎非醇、松属素、柯因、高良姜素、苯甲酰肉桂酯以河南蜂胶含量最高；芹菜素、异鼠李素以辽宁蜂胶含量最多；山东蜂胶含有最多的鼠李素。

Composition Analysis in Ethanol Extracts of Propolis Samples from Northern China

The purpose of this study was to compare the contents of total phenols, total flavonoids and individual components in ethanol extracts of propolis samples collected from Northern China (Beijing and 10 provinces). The content of total flavonoids and total phenols were determined by NaNO2-Al(NO3)3 method and Folin-Ciocalteau method, respectively. Quantitative and qualitative HPLC analysis of phenols in propolis extracts was carried out by comparison with standard references for geographic cluster analysis of propolis samples. The results showed that the contents of total flavonoids and total phenols of propolis samples varied with geographic origin. The highest contents of caffeic acid, kaempferol, pinocembrin, chrysin, galangin and benzyl cinnamate were found in propolis samples from Henan province; propolis samples from Liaoning province were the richest in apigenin and isorhamnetin; propolis samples from Shandong province showed the highest content of rhamnetin.