黄秋葵多糖的提取、分离及其体外结合胆酸盐能力的分析

探讨黄秋葵结合胆酸的有效组分的分离纯化，通过水提醇沉提取黄秋葵粗多糖(raw polysaccharide，RPS)，经DEAE-纤维素阴离子交换层析柱分离得到3种多糖洗脱组分E1、E2和E3；对3种组分的体外结合胆酸能力进行分析。结果显示： 组分E1和E2有较高的胆酸结合能力，分别为参照药物消胆胺的10.82%和10.60%，黄秋葵多糖体外结合胆酸作用较强。

Extraction,Purification and Bile Acid-binding Capacity in vitro of Polysaccharides from Okra

In order to investigate active components having the ability to bind bile acid from okra, Abelmoschus esculentus (L.) Moench, raw polysaccharide (RPS) was obtained from the fresh fruits of okra by water extraction and ethanol precipitation. RPS was further purified by DEAE anion exchange chromatography to obtain fractions E1, E2 and E3. Their bile acid-binding capacities in vitro were determined. The results indicated that fractions E1 and E2 presented different bile acid binding capacities, which accounted for 10.82% and 10.60% of that of cholestyramine, respectively. These investigations provide some significant references for exploring and developing okra as health food or functional additives.