| 高效液相色谱法测定食品中亮蓝同分异构体 |
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| 引用本文： | 刘立萍,黄蔚,唐序文,彭高英,刘国,陈琳巧.高效液相色谱法测定食品中亮蓝同分异构体[J].食品科学,2012,33(8):221-224. |
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| 作者姓名： | 刘立萍 黄蔚 唐序文 彭高英 刘国 陈琳巧 |
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| 作者单位： | 1.怀化市质量技术监督局产商品质量监督检验所
2.怀化市中方县质量技术监督局质量检验及计量检定所 |
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| 摘 要： | 建立高效液相色谱法检测食品中亮蓝的新方法，初步证实食品中亮蓝主要为3种异构体的混合物。用聚酰胺层析柱净化，高效液相色谱法分离。色谱柱为Inertsil ODS-SP C18 (150mm×4.6mm，5μm)，流动相为甲醇-0.02mol/L乙酸铵溶液，检测波长629nm。加标回收率为91.1%～94.7%。
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| 关 键 词： | 亮蓝同分异构体 高效液相色谱 食品 |
Determination of Brilliant Blue Isomers in Food by High Performance Liquid Chromatography |
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| LIU Liping,HUANG Wei,TANG Xu-wen,PENG Gao-ying,LIU Guo,CHEN Lin-qiao.Determination of Brilliant Blue Isomers in Food by High Performance Liquid Chromatography[J].Food Science,2012,33(8):221-224. |
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| Authors: | LIU Liping HUANG Wei TANG Xu-wen PENG Gao-ying LIU Guo CHEN Lin-qiao |
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| Affiliation: | (1. Institute of Product and Commodity Quality Inspection and Supervision, Huaihua Quality and Technical Supervision Bureau,
Huaihua 418000, China；2. Institute of Quality Inspection and Measurement Calibration,
Zhongfang County Bureau of Quality and Technical Supervision, Zhongfang 418005, China) |
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| Abstract: | A new analytical method was developed for the determination of brilliant blue in food by high performance liquid chromatography(HPLC).It was preliminarily confirmed that the presence of brilliant blue in food was mixtures of its three isomers.Sample extracts were purified by polyamide column chromatography and separated on an Inertsil ODS-SP C18(150 mm × 4.6 mm,5 μm) column using a mobile phase made up of methanol and 0.02 mol/L ammonium acetate solution.The detection wavelength was set as 629 nm.The spike recovery rates across three spike levels were in the range of 91.1%-94.7%.The method was simple and accurate. |
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| Keywords: | brilliant blue isomers high performance liquid chromatography(HPLC) food |
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