加速溶剂萃取-高效液相色谱法测定方便面中栀子黄色素

建立一种检测方便面中栀子黄色素的加速溶剂萃取-高效液相色谱方法。采用加速溶剂萃取法萃取方便面,以甲醇为萃取剂,在1 500 psi和50℃静态循环提取2次,用乙腈饱和的正己烷溶液除脂。采用XBridge~(TM) C_(18)(4.6 mm×250 mm,5μm)色谱柱,以乙腈-0.1%甲酸溶液为流动相,藏花素和藏花酸在二极管阵列检测器中的检测波长为441 nm和427 nm进行分离分析。结果表明,藏花素和藏花酸在1.0~20μg/mL范围内具有良好的线性关系(r0.999 6);平均回收率(n=6)在83.64%~94.82%之间;相对标准偏差为0.92%~2.13%,方法的定量限和检出限分别为0.5~1.0μg/g和0.05~0.20μg/g,本方法快速简便、自动化程度高、准确可靠,是一种适合测定方便面中栀子黄色素的方法。

Determination of Gardenia Yellow in Instant Noodle by Accelerated Solvent Extraction and High Performance Liquid Chromatography

A method was developed for the determination of gardenia yellow in instant noodle by accelerated solvent
extraction (ASE) and high performance liquid chromatography (HPLC). The crushed samples were extracted twice using
ASE with methanol at 50 ℃ and 1 500 psi, followed by defatting using n-hexane saturated with acetonitrile. Then the
gardenia yellow were eluted on XBridgeTM C18 with a mobile phase composed of acetonitrile and 0.1% formic acid solution,
and detected by photo-diode array (PDA) detector at 441 nm for crocin and 427 nm for crocetion. The developed method had
a linear range of 1.0?20 μg/mL for the two analytes with good correlation coefficients (r > 0.999 6). The mean recoveries
were 83.64%?94.82%, with relative standard deviation (RSD) of 0.92%?2.13% (n = 6). The limit of quantitation (LOQ)
and the limit of detection (LOD) of this method for gardenia yellow were 0.5?1.0 μg/g and 0.05?0.2 μg/g, respectively. The
method proved to be quick, simple, automated, reliable, accurate, and suitable to detect gardenia yellow in instant noodle.