| 两种特异性氰戊菊酯人工抗原的合成与鉴定 |
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| 引用本文： | 熊振宇,杨艳燕,李培武,张奇,张文,丁小霞.两种特异性氰戊菊酯人工抗原的合成与鉴定[J].食品科学,2011(7). |
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| 作者姓名： | 熊振宇 杨艳燕 李培武 张奇 张文 丁小霞 |
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| 作者单位： | 湖北大学生命科学学院;中国农业科学院油料作物研究所;农业部油料作物生物学重点开放实验室;农业部油料及制品质量监督检验测试中心; |
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| 基金项目： | 国家自然科学基金项目(30800771); 国家现代农业(油菜)产业技术体系建设专项(nycytx-005); 国家科技支撑计划项目(2010BAD01B07;2011BAD02D02); 武汉市晨光青年计划项目(200950431224); 武汉市科技研发中心平台项目(200820337086) |
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| 摘 要： | 选择化学结构具有典型研究意义的氰戊菊酯(fenvalerate,Fen)为研究对象,利用有机化学合成法在氰戊菊酯分子的一端分别引入两种不同连接长度的连接臂。通过核磁共振(1H-NMR)、液相色谱-质谱联用仪(LC-MS-MS)鉴定,成功合成两种半抗原Fen1和Fen2,并与牛血清蛋白(bovine serum albumin,BSA)偶联合成了两种氰戊菊酯免疫特异性人工抗原Fen1-BSA、Fen2-BSA,经紫外分光光度仪扫描,人工抗原的紫外图谱相对于半抗原和载体蛋白有明显差异,测得偶联比分别约为9:1和10:1,经SDS-PAGE电泳检测表明偶联成功。
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| 关 键 词： | 氰戊菊酯 半抗原 人工抗原 合成 |
Synthesis and Identification of Two Types of Specific Artificial Antigen for Fenvalerate |
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| XIONG Zhen-yu,YANG Yan-yan,LI Pei-wu,,ZHANG Qi,ZHANG Wen,DING Xiao-xia.Synthesis and Identification of Two Types of Specific Artificial Antigen for Fenvalerate[J].Food Science,2011(7). |
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| Authors: | XIONG Zhen-yu YANG Yan-yan LI Pei-wu ZHANG Qi ZHANG Wen DING Xiao-xia |
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| Affiliation: | XIONG Zhen-yu1,YANG Yan-yan1,LI Pei-wu2,3,4,ZHANG Qi2,ZHANG Wen2,DING Xiao-xia2,4(1.Faculty of Life Sciences,Hubei University,Wuhan 430062,China,2.Oil Crops Research Institute,Chinese Academy of Agricultural Sciences,3.Key Laboratory of Oil Crops Biology,Ministry of Agriculture,4.Quality Inspection and Test Center for Oilseeds and Products,China) |
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| Abstract: | Fenvalerate,with a typical chemical structure,was selected as the research object in this study.Two types of haptens were synthesized through separately introducing two space arms with different connecting lengths to one end of fenvalerate by organic synthesis.1H-NMR and LC-MS-MS identifications demonstrated the successful synthesis of haptens,which were designated as Fen 1 and Fen 2.Fen 1 and Fen 2 were separately coupled to bovine serum albumin(BSA),and two kinds of specific immunogens were obtained and n... |
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| Keywords: | fenvalerate hapten artificial antigen synthesis |
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