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| 采用基因敲除手段降低啤酒酵母蛋白酶A表达的研究 | |
| 引用本文： | 周建中,王德良,王忠民,赵丽娟,葛邦国.采用基因敲除手段降低啤酒酵母蛋白酶A表达的研究[J].酿酒,2006,33(1):62-64. |
| 作者姓名： | 周建中 王德良 王忠民 赵丽娟 葛邦国 |
| 作者单位： | 1. 新疆农业大学食品学院,乌鲁木齐,830052 2. 中国农业大学食品学院,北京,10009;中国食品发酵工业研究院酿酒工程部,北京,100027 3. 沈阳农业大学食品学院,沈阳,110161 |
| 摘 要： | 采用醋酸锂转化法,将一段目的基因转入到酵母体内,破坏酵母PEP4基因的表达。通过对目的基因的扩增和羧肽酶Y酶活检测验证基因敲除情况,并通过传代抗性试验与传代发酵稳定性实验验证突变株遗传稳定性良好。 |
| 关 键 词： | 酿酒酵母 基因敲除 羧肽酶Y酶活性检测 |
| 文章编号： | 1002-8100（2006）01-0062-03 |
| 收稿时间： | 2005-10-26 |
| 修稿时间： | 2005-10-26 |
Study on Disruption of Saccharomyces cerevisiae PEP4 Gene |
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| ZHOU Jian-zhong,WANG De-liang,WANG Zhong-min,ZHAO Li-juan,GE Bang-guo.Study on Disruption of Saccharomyces cerevisiae PEP4 Gene[J].Liquor Making,2006,33(1):62-64. | |
| Authors: | ZHOU Jian-zhong WANG De-liang WANG Zhong-min ZHAO Li-juan GE Bang-guo |
| Affiliation: | 1. Xinjiang Agricultural University, Wulumuqi,830052, China;2. College of Food Science, China Agricultural University, Beijing, 100094, China ; 3. China National Research Instintue of Food and Fermentation Industries, Beijing, 100027, China; 4. Shenyang Agricultural University, Shenyang, 110034, China |
| Abstract: | In this paper,we transform a gene into yeast genome so as to disrupt PEP4 gene by LiAc method.The mutant gene can be identified by PCR and testing enzyme activity of CPY.The mutant strain inheritance and ferment stability is well. |
| Keywords: | Saccharomyces cerevisiae gene knock-out CPY activity test |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |