高效液相色谱检测银杏茶中总黄酮含量

采用正交试验法优选银杏茶中黄酮类化合物的最佳提取条件为25%HCl添加量4 mL,提取温度100℃,提取时间90 min。分析方法采用Eclipse XDB C18(250 mm×4.6 mm,5μm)色谱柱,流动相为甲醇-0.4%磷酸溶液(体积比60∶40),流速1.0 mL/min,柱温30℃,检测波长为360 nm。槲皮素、山柰酚、异鼠李素在浓度20μg/mL～100μg/mL范围内线性良好,检出限分别为5.0、8.6、6.7 mg/kg。相对标准偏差(RSD)小于5%(n=6)。本法准确、快速、重现性好,可用于银杏茶的质量控制。

Determination of Flavonoids in Ginkgo Biloba Tea by HPLC

The optimum extracting technology of flavonodis in ginkgo biloba tea was investigated by using the factorial and orthogonal design.The optimal conditions included 25 % HCl added 4 mL,extraction temperature 100 ℃,and extraction 1.5 h for each times.The separation was performed on Eclipse XDB C18 column(250 mm×4.6 mm,5 μm).The mobile phase was methanol-0.4 % phosphoric acid(60∶40,volumn ratio)with a flow rate of 1.0 mL/min.The detection wavelength was set at 360 nm and the column teperature was 30 ℃.A linear calibration curve of quercetin,isorhamnetin and keampferol was obtained in the concentration range of 20 μg/mL-100 μg/mL.The qualification limit were 5.0,8.6,6.7 mg/kg.The relative standard deviations(RSDs)were below 5 %(n=6).The method was simple,accurate and can be used for quality control of ginkgo biloba tea.