| 烟叶表面高效降解蛋白质芽孢杆菌的分离筛选及其鉴定 |
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| 引用本文： | 冯志珍,陈太春,胡喜怀,郭志刚,安德荣.烟叶表面高效降解蛋白质芽孢杆菌的分离筛选及其鉴定[J].中国烟草学报,2012,18(1):101-105. |
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| 作者姓名： | 冯志珍 陈太春 胡喜怀 郭志刚 安德荣 |
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| 作者单位： | 1. 西北农林科技大学植物保护学院,陕西省农业分子生物学重点实验室,杨凌712100
2. 陕西中烟工业有限责任公司,西安,710065 |
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| 基金项目： | 国家“863特殊生物资源开发和利用项目”(2007AA021503);陕西中烟工业公司生物发酵增香减害项目(2010182) |
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| 摘 要： | 利用芽孢杆菌耐高温的特性,从烟叶表面分离筛选并得到1株可高效降解蛋白质的菌株F-Y-11,其蛋白质降解圈直径与菌落直径比值(HC值)为5.50,蛋白酶活力为25.67 U.通过形态学特征及生理生化特征鉴定菌株F-Y-11为芽孢杆菌属.将其制成菌剂对烟丝进行发酵,测得蛋白质降解率为29.32％.
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| 关 键 词： | 烟叶 生物降解 芽孢杆菌 |
Isolation,screen and identification of Bacillus with high proteolytic activity from tobacco leaf surfaces |
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| FENG Zhi-zhen
,
CHEN Tai-chun
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HU Xi-huai
,
GUO Zhi-gang
,
AN De-rong.Isolation,screen and identification of Bacillus with high proteolytic activity from tobacco leaf surfaces[J].Acta Tabacaria Sinica,2012,18(1):101-105. |
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| Authors: | FENG Zhi-zhen CHEN Tai-chun HU Xi-huai GUO Zhi-gang AN De-rong |
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| Affiliation: | 1 College of Plant Protection,Shaanxi Key Laboratory of Molecular Biology for Agriculture, Northwest A&F University,Yangling 712100 Shannxi,China; 2 China Tobacco Shaanxi Industrial Co.Ltd.,Xi’an 710065,Shannxi,China |
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| Abstract: | A strain called F-Y-11 was isolated and screened from tobacco leaf surfaces.Its HC value,i.e.ratio of colony diameter to protein degradation circle diameter,is 5.50 and proteolytic activity 25.67U.F-Y-11 strain was identified as bacillus by morphological,physiological and biochemical characteristics.Bacillus agent was prepared to ferment cut tobacco and resulted in a 29.32% of protein degradability under certain conditions. |
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| Keywords: | tobacco leaf bio-degradation bacillus |
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