| 马铃薯渣高效降解菌的紫外诱变选育及发酵试验 |
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| 引用本文： | 马振贵,马伟超,蒲建平,安建平.马铃薯渣高效降解菌的紫外诱变选育及发酵试验[J].中国酿造,2011(1). |
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| 作者姓名： | 马振贵 马伟超 蒲建平 安建平 |
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| 作者单位： | 兰州交通大学,化学与生物工程学院,甘肃兰州730070 |
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| 基金项目： | 甘肃省科技厅资助项目(090NKCE081); 天水师范学院中青年教师科研资助项目(TSA0918) |
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| 摘 要： | 以选育马铃薯渣高效降解菌为目的,对康宁木霉进行紫外诱变,以致死率在80%~90%之间的菌株进行初筛,通过羧甲基纤维素钠刚果红筛选平板复筛,选育出两株具有较高酶活的菌株,并命名为P12、P13。通过诱变,完全培养基中P12的纤维素酶活(CMCA)及滤纸酶活(FPA)分别提高了1.45倍、1.738倍,P13的羧甲基纤维素酶活(CMCA)及滤纸酶活(FPA)分别提高了1.4倍和1.667倍。并通过发酵试验,发酵培养基中CMC酶活分别是初始菌株的1.72倍和1.84倍。P12和P13具有良好的降解马铃薯渣的能力。
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| 关 键 词： | 康宁木霉 紫外诱变 酶活 发酵试验 |
UV mutagenesis in efficient degradation strains of potato pulp and fermentation |
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| MA Zhengui,MA Weichao,PU Jianping,AN Jianping.UV mutagenesis in efficient degradation strains of potato pulp and fermentation[J].China Brewing,2011(1). |
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| Authors: | MA Zhengui MA Weichao PU Jianping AN Jianping |
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| Affiliation: | MA Zhengui,MA Weichao,PU Jianping,AN Jianping*(School of Chemical and Biological Engineering,Lanzhou Jiaotong University,Lanzhou 730070,China) |
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| Abstract: | Trichoderma koningii is mutated by UV for the selection of efficient degradation strains of potato pulp.The treated strains with a death rate of 80%~90% were preliminarily screened,and then were secondly screened by CMC Congo red agar plate,by which two strains with higher activity on degradation were isolated and named as P12 and P13.After mutation,the CMC activity(CMCA) and filter paper activity(FPA) of cellulase in P12 were improved 1.45 times and 1.738 times respectively in full media,while the CMCA and... |
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| Keywords: | Trichoderma koningii UV mutagenesis enzyme activity fermentation |
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