蓝莓果渣花色苷大孔树脂纯化工艺研究

以蓝莓果渣为原料，利用大孔树脂分离纯化蓝莓果渣花色苷。对比了D101和AB-8两种不同极性的大孔树脂静态吸附和解吸效果。结果表明，AB-8型大孔树脂吸附率和解吸率分别为88.5%、64.7%；D101型大孔树脂吸附率和解吸率分别为86.7%、61.2%，AB-8型大孔树脂吸附率和解吸率均优于D101型大孔树脂，故选用AB-8型大孔树脂对蓝莓果渣进行纯化试验。AB-8型大孔树脂最佳吸附和解吸条件为吸附平衡时间4 h，解吸平衡时间4 h，花色苷溶液pH 3.0，解吸液pH 3.0，解吸液乙醇体积分数60%，上样质量浓度1 mg/mL，上样流速1 mL/min，洗脱流速1 mL/min。纯化后蓝莓果渣花色苷色价约为纯化前的3倍，糖和蛋白质等杂质大幅降低，纯度有了较大提高。

Purification process of blueberry pomace anthocyanin with macroporous resin

Using blueberry pomace as raw material, anthocyanins from blueberry pomace were separated and purified by macroporous resin. The static adsorption and desorption effects of macroporous resins D101 and AB-8 with different polarity were compared. The results showed that the adsorption rate and desorption rate of AB-8 type macroporous resin were 88.5% and 64.7%, respectively; The adsorption rate and desorption rate of macroporous resin D101 were 86.7% and 61.2%, respectively, and the adsorption and desorption rates of AB-8 macroporous resin was better than macroporous resin D101. Therefore, macroporous resin AB-8 was selected to carry out purification experiments of blueberry pomace. The optimal adsorption and desorption conditions of macroporous resin AB-8 were as follows: adsorption equilibrium time 4 h, desorption equilibrium time 4 h, anthocyanin solution pH 3.0, desorption solution pH 3.0, desorption solution ethanol volume fraction 60%, loading mass concentration 1 mg/ml, loading flow rate 1 ml/min, and elution flow rate 1 ml/min. The color value of anthocyanins from blueberry pomace after purification was about 3 times that of before purification, the impurities such as sugar and protein were greatly reduced, and the purity was greatly improved.