UPLC-MS/MS测定啤酒中茚嗪氟草胺及其代谢物残留量

该实验建立了基于分散固相萃取结合超高效液相色谱-串联质谱技术（UPLC-MS/MS）对啤酒中的茚嗪氟草胺及其3种代谢物残留量的测定方法。样品经乙腈提取后进行盐析分层，离心后乙腈层用C18固相萃取剂净化后，于电喷雾离子源正离子（ESI+）模式下采集碎片信息。结果表明，茚嗪氟草胺及其3种代谢物的基质曲线在1.0～50.0 μg/L的质量浓度内呈良好线性关系，相关系数（R2）均大于0.999，平均加标回收率为85.4%～98.1%，精密度试验结果的相对标准偏差（RSD）为2.29%～4.55%。方法检出限（LOD）和定量限（LOQ）分别为2.0 μg/kg和5.0 μg/kg。该方法快速高效，回收率、灵敏度高，重复性好，可有效测定啤酒中茚嗪氟草胺及其代谢物的残留量。

Determination of indaziflam and its metabolites residues in beer by UPLC-MS/MS

A dispersive solid-phase extraction combined with ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was established for the determination of indaziflam and its three metabolites residues in beer. The samples were extracted by acetonitrile, then stratified by salting. After centrifugation, the acetonitrile phase was cleaned up by C18 solid-phase extractant, and the fragment information was collected by electron spray ionization (ESI+). The results showed that the matrix curves of indaziflam and its three metabolites had good linearity within the mass concentrations of 1.0-50.0 μg/L, the correlation coefficient (R2) were greater than 0.999, the average recoveries were 85.4%-98.1%, and the relative standard deviation (RSD) of precision test results were 2.29%-4.55%. Limits of detection (LOD) and limits of quantitation (LOQ) of the method were 2.0 μg/kg and 5.0 μg/kg, respectively. The method was fast and efficient with high recovery, sensitivity and repeatability, which could effectively determine indaziflam and its metabolites residues in beer.