3种脂肪氧合酶酶活测定方法

采用量压法、分光光度法和KI-淀粉法测定了一系列经不同程度热处理的脂肪氧合酶酶活力.分析了3种方法的优缺点和相关性，结果表明相关性良好，其中，量压法具有最广泛的酶液适用性，分光光度法灵敏度最高，KI-淀粉法更适合于工业化应用.

Comparison of Three Different Methods for Determination of Lipoxygenase Activity

Three different methods for determination of lipoxygenaseactivity,known as manometric method, spectrphotometric and KI-starch method, were applied to a series of enzyme extractions with various degrees of heat-treatment at 70 ℃. Advantage and disadvantage of each method, as well as the correlation among the three,were discussed. The result showed that all the three methods were reliable and highly correlated, especially for the spectrophotometic and manometric methods. Nevertheless, each has its own specialty,that is, manometric technique has a wide rang of applicability, spectrophotometic method is utmost sensitive, and KI-starch method is more suitable for use by industry.