红酵母类胡萝卜素发酵助剂的筛选及应用

研究了几种促进剂和前体对红酵母RY－98类胡萝卜素发酵的影响，从中选出了番茄汁、花生油和核黄素3种对红酵母生长及类胡萝卜素合成具有显著促进作用的发酵助剂，并确定了适宜的用量。应用试验和发酵过程动态分析表明，这3种发酵助剂增产效果明显。当同时添加番茄汁3mL/L、花生油1.2mL/L和核黄素3.5mg/L时，菌体量、类胡萝卜素质量分数和产量可分别比对照组提高39.4%、32.8%和85.1%，且对发酵过程菌体生长及生理代谢规律无不良影响。

Studies on the Promotor of Carotenoids Fermentation by Rhodotorula

The effects of some additives on the growth, carotenoid content and yield of Rhodotorula RY 98 were studied .The results showed that the suitable concentrations of tomato juice, peanut oil and VB 2 could obviously increase the biomass and the biosynthesis of carotenoids from this strain. When the tomato juice of 3 mL/L, peanut oil of 1.2 mL/L and VB 2 of 3.5 mg/L were added to the media, the cell biomass, carotenoid content in the cells and the yield of carotenoids could be increased by 39.4%?32.8% and 85.1%, respectively. In terms of the results, tomato juice, and peanut oil and VB 2 belong to three kinds of very important promotor to carotenoid fermentation by Rhodotorula.