| 向日葵抗锈病基因同源序列的克隆与分析 |
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| 引用本文： | 林晓红,;景岚,;康振生.向日葵抗锈病基因同源序列的克隆与分析[J].中国油料,2014(4):508-512. |
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| 作者姓名： | 林晓红 ;景岚 ;康振生 |
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| 作者单位： | [1]内蒙古农业大学农学院,内蒙古呼和浩特010019; [2]西北农林科技大学、旱区作物逆境生物学国家重点实验室,陕西杨凌712100 |
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| 基金项目： | 国家自然科学基金（31060235,31360422）;旱区作物逆境生物学国家重点实验室开放课题;国家向日葵现代产业技术体系（CARS-16） |
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| 摘 要： | 向日葵锈病严重影响向日葵的产量。为了找到抗锈病相关基因，根据已知NBS-LRR型抗病基因保守结构域P-loop和GLPL设计简并引物，以接菌的抗病向日葵品种CM29叶片的cDNA为模板进行扩增。通过克隆转化得到10个具有连续开放阅读框的抗病基因同源序列（RGA），经系统进化分析将其分为TIR-NBS-LRR和non-TIR-NBS-LRR两种类型。对其氨基酸序列进行多重序列比对，结果显示所获得的RGA具有典型的NBS-LRR型抗病基因保守结构域，即P-loop、kinase-2a、kinase-3a和GLPL结构。运用BLASTX分析这些结果，表明其RGA与已知的抗病基因相应保守区域的同源性为18．1％～51．1％，说明它们可能与抗病功能基因具有密切联系。
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| 关 键 词： | 向日葵 锈病 抗病基因同源序列(RGA) 克隆 |
Cloning and analysis of rust resistance gene analogs of sunflower |
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| Affiliation: | LIN Xiao-hong, JING Lan, KANG Zhen-sheng ( 1. College of Agronomy, Inner Mongolia Agricultural University, Hohhot 01 O019, China ; 2. State Key Laboratory of Crop Stress Biology in Arid Areas ,Northwest A＆F University, Yangling 712100, China ) |
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| Abstract: | Sunflower （Helianthus annuus）rust restricts growth and production severely.In order to find resist-ance genes of sunflower to rust disease,degenerated primers were designed based on conserved regions of NBS -LRR genes,P-loop and GLPL,to clone the analogs from infected resistant cultivar CM29 .By phylogenetic analy-sis,10 cloned resistance gene analogs with continuous open reading frame belonged to TIR-NBS-LRR type and non-TIR-NBS -LRR.Multiple sequence alignment of amino acid showed these RGAs had typical domains of NBS-LRR resistance genes,including P-loop,kinase-2a,kinase-3a and GPLP.These RGAs showed great homology with the known resistance genes by BLASTX analysis,indicating the RGCs were highly related to resist-ance genes in sunflower. |
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| Keywords: | Helianthus annuus Puccinia helianthi Schw NBS -LRR Resistance gene analogs Clone |
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