| 牛初乳中乳铁蛋白的分离及纯化 |
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| 引用本文： | 凌雪萍,庞广昌,李国强.牛初乳中乳铁蛋白的分离及纯化[J].食品工业科技,2002,23(12):40-41. |
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| 作者姓名： | 凌雪萍 庞广昌 李国强 |
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| 作者单位： | 天津商学院生物工程系,天津,300134 |
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| 摘 要： | 乳铁蛋白(Lactoferrin,简称Lf)作为一种糖蛋白,主要存在于哺乳动物的各种外分泌物中,如乳汁、眼泪、唾液等,乳汁尤其是初乳中Lf的含量很高犤1犦。本文通过盐析法和有机溶剂沉淀法从牛初乳中分离出乳铁蛋白,并应用酶联免疫法鉴定各步骤中分离的乳铁蛋白,获得的乳铁蛋白的相对分子量为75,000Da。
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| 关 键 词： | 乳铁蛋白 盐析 有机溶剂沉淀 酶联免疫 |
| 文章编号： | 1002-0306(2002)12-0040-02 |
| 修稿时间： | 2002年7月2日 |
Isolation and purification of Lactoferrin from bovine colostrums |
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| Ling Xueping et al.Isolation and purification of Lactoferrin from bovine colostrums[J].Science and Technology of Food Industry,2002,23(12):40-41. |
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| Authors: | Ling Xueping |
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| Affiliation: | Ling Xueping et al |
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| Abstract: | The lactoferrin,an iron -binding g lycoprotein,is presented in numerous exocrine secretions of mammals,including milk,tears,saliva,and so on.Milk,especially colostrum contains lactoferrin at a much higher level than others.In this paper,Lf was separated and purified by salt-out and organic reagents precipitation from bovine colostrums,and it was measured by enzyme -linked immunosorbent assay (ELISA).For the acquired lactoferrin,the molecular weight was estimated at 75,000Da,and the purity was about 45%. |
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| Keywords: | lactoferrin (Lf) salt-out org anic reag ent precipitation ELISA |
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