鸡枞菌粗蛋白超声辅助提取工艺优化及其抗氧化活性

以鸡枞菌为原料,以蛋白提取率为指标,采用单因素实验和正交实验,优化了超声波辅助提取鸡枞菌粗蛋白的工艺条件,并探讨了鸡枞菌粗蛋白对ABTS^+·、DPPH·、O_2^-·自由基的清除能力。结果表明:鸡枞菌粗蛋白提取的最佳条件为超声功率400 W、超声温度70℃、超声时间1.5 h、p H11、料液比1∶25 g/m L,此条件下蛋白质含量为86.525%±1.315%,蛋白提取率为67.322%±1.028%。鸡枞菌粗蛋白对ABTS^+·、DPPH·和O_2^-·三种自由基均有清除作用,其IC₅₀分别为46.322、61.176和151.250 mg/L,但自由基的清除能力明显低于同浓度VC的清除能力。因此,超声波辅助适用于鸡枞菌粗蛋白的提取,所得粗蛋白具有抗氧化活性,该研究可为鸡枞菌高值化加工与利用提供理论依据。

Optimization of Ultrasonic-assisted Extraction Process of Crude Protein from Termitomyces albuminosus and Its Antioxidant Activity

Using Termitomyces albuminsous as experimental materials,the optimum conditions of ultrasonic-assisted extraction from Termitomyces albuminsous with the extraction rate of protein as index were studied through the method of single factor and orthogonal experiments.The crude protein’s antioxidant activities of ABTS^+·,DPPH·and O2^-·radical scavenging assays were also investigated in this paper. The results showed that the optimum extraction conditions were ultrasonic power 400 W,ultrasonic temperature 70 ℃,ultrasonic time 1.5 h,pH11 and solid-to-solvent ratio 1 ∶ 25 g/m L,respectively. The extraction rate of crude protein could reach up to 67.322%± 1.028% and the content of protein was 86.525%± 1.315% under these conditions.Termitomyces albuminosus crude protein had a certain antioxidant activity.The scavenging ability of ABTS^+·,DPPH·and O2^-·free radical IC50( half maximal inhibitory concentration) value were separately 46.322,61.176 and 151.250 mg/L.The free radical scavenging capacity of Termitomyces albuminosus protein was lower than that of VC’s ability to remove them.Therefore,the ultrasonic-assisted technology was suitable for crude protein extraction from Termitomyces albuminosus and the protein had antioxidant activity.The study provided a theoretical basis for high value processing of Termitomyces albuminosus.