藜麦糠抗氧化肽的超滤分离及其体外抗氧化活性

为了大批量生产抗氧化活性最好的藜麦糠抗氧化肽,采用风味酶与碱性蛋白酶组合对藜麦糠蛋白进行酶解,制备酶解液。利用超滤技术分离纯化藜麦糠抗氧化肽,并评价分离得到的不同分子质量段抗氧化肽的体外抗氧化活性,优选出抗氧化活性最好的抗氧化肽。以膜通量为指标,压力、时间和滤液质量分数为3个工艺参数,通过单因素和正交实验,探究超滤分离截留分子量为10 ku的藜麦糠抗氧化肽的最佳工艺条件。结果表明,不同分子量段的超滤组分具有不同的抗氧化活性,其中分子量为5~10 ku的抗氧化肽较其他分子量范围的超滤组分的抗氧化活性最好。藜麦糠抗氧化肽的最佳超滤条件为压力0.16 MPa,时间20 min,滤液质量分数2%,在此条件下得到膜通量为(41.22±0.50) L/m2·h。由此可实现5~10 ku的抗氧化肽的大量生产。

The separation of the antioxidant peptide from quinoa chaff by ultrafiltration and its antioxidant activity in vitro

In order to acheive mass production of antioxidant peptides from quinoa chaff with the best antioxidant activity,the enzymatic hydrolysate from quinoa chaff protein was made through enzymolysis used flavor enzyme and alkaline enzyme. The antioxidant peptide of quinoa chaff with different molecular mass were purified by ultrafiltration technology. Antioxidant activity in vitro were measured and evaluated to optimize and select in order to get the antioxidant peptides with the best antioxidant activity. Took membrane flux as the index,the stress,time and filtrate quality score as 3 process parameters. The optimum technological conditions of the ultrafiltration of 10 ku ultrafiltration separation of antioxidant peptides from quinoa chaff were studied by the combination of single factor and orthogonal test. The results showed that,the ultrafiltration components with different molecular weights had different antioxidant activity,of which the antioxidant peptide of 5~10 ku had better antioxidant activity than the others ultrafiltration components of different molecular weights.The optimal ultrafiltration conditions of antioxidant peptides from quinoa chaff were pressure 0.16 MPa,20 min time,2% of filtrate quality score,and the maximum amount of membrane flux in the condition was(41.22±0.50) L/m2·h.Thus,the production of antioxidant peptides from 5~10 ku can be achieved.