| 比色法测定原料乳中乳糖含量研究 |
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| 引用本文： | 蒋珍菊,林芳栋.比色法测定原料乳中乳糖含量研究[J].食品科技,2012(6):299-303. |
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| 作者姓名： | 蒋珍菊 林芳栋 |
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| 作者单位： | 西华大学生物工程学院;河南省中牟县卫生局 |
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| 基金项目： | 四川省重点学科建设项目;四川省教育厅科研基金项目(08ZC011) |
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| 摘 要： | 将比色法引入原料乳中乳糖含量的测定,在现有基础上考察了比色法在原料乳中测得乳糖含量的反应温度、反应时间、沉淀剂使用量、精密度、重复性等。由实验结果可知,比色法的线性回归方程为y=4.3705x-0.0038,相关系数R2=0.9992,曲线的相关性很好;精密度为0.551%,重复性为1.809%,加标回收率为100.434%,乳糖盲样测定结果也与实际相符,用该方法进行原料乳中乳糖的测定是切实可行的。
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| 关 键 词： | 比色法 原料乳 乳糖含量 |
The research on lactose content in the raw milk by colorimetry |
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| Affiliation: | JIANG Zhen-ju1,LING Fang-dong2(1.School of Bioengineering,Xihua University,Chengdu 610039;2.Henan Province Zhongmou County Public Health Bureau,Zhengzhou 4515450) |
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| Abstract: | The lactose content in the raw milk by colorimetry was introduced in the paper.On the existing basis,the contrast color method of reaction temperature,reaction time,amount of precipitation agent,precision,repeatability and so on were reachered.The precision of the Lane-Eynon was 0.551%,the repeatability was 1.809%,the average recovery was 100.434%,the result of the milk blind samples was consistent with the actual,so it is feasible to determine the content of lactose in the raw milk. |
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| Keywords: | colorimetry raw milk lactose content |
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