高效液相-紫外法检测三孢布拉霉菌发酵液中2-氨基-6-甲基吡啶

建立高效液相色谱-紫外法对三孢布拉霉菌发酵液中2-氨基-6-甲基吡啶含量检测的方法。采用AlltimaC18(250mm×4.6mmi.d.,5μm)色谱柱,以甲醇:水:1%乙酸=85:10:5(v/v/v)为流动相,流速为1.0mL/min时,2-氨基-6-甲基吡啶及其他杂质得到良好分离,紫外光谱231nm检测。在该色谱条件下,2-氨基-6-甲基吡啶浓度5～500mg/L时,其峰面积与相应的浓度线性关系良好(R2=0.9994),回收率99.76%～101.4%,精密度RSD<2.17%。该方法简便、快速、准确可靠,为三孢布拉霉菌发酵液中2-氨基-6-甲基吡啶的检测和含量控制提供了可靠的分析方法。

Determination of 2-amino-6-methylpyridine in Blakeslea trispora fermentation broth by HPLC-UV method

A high performance liquid chromatography-ultraviolet spectrometry(HPLC-UV) method has been developed for determination of 2-amino-6-methylpyridine in Blakeslea trispora fermentation broth.2-amino-6-methylpyridine and other relative impurities was achieved on a Alltima C18(250 mm×4.6 mm i.d.,5 μm) by using methanol-water-1% aqueous acetic acid solution(85:10:5,v/v/v) as the mobile phase at a flow rate of 1.0 mL/min.Detection was operated by UV absorption at a wavelength of 231 nm.The calibration curves were showed good linearity(r 2 =0.9994) within the concentration range of 5.0～500 mg/L for 2-amino-6-methylpyridine.The average recoveries were ranged from 99.76%～101.4%.And the standard deviation was less than 2.17%.The method was simple and accurate for the determination of 2-amino-6-methylpyridine in Blakeslea trispora fermentation broth.