| Protamex蛋白酶水解大豆蛋白研究 |
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| 引用本文： | 赵谋明,吴建中,欧仕益,付亮,汪勇.Protamex蛋白酶水解大豆蛋白研究[J].食品与发酵工业,2004,30(7):82-86. |
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| 作者姓名： | 赵谋明 吴建中 欧仕益 付亮 汪勇 |
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| 作者单位： | 1. 华南理工大学食品与生物工程学院,广州,510641
2. 暨南大学食品科学系,广州,510632 |
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| 基金项目： | 国家“十五”招标攻关项目资助 (No .2 0 0 1BA5 0 1A0 2 )，广东省“十五”攻关农产品加工重大专项资助 (No.A2 0 30 1 ) |
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| 摘 要： | 研究了Protamex酶对大豆蛋白的有限水解作用 ,分析了酶浓度、pH值、温度、底物浓度、反应时间等因素对大豆蛋白酶水解的影响 ,给出了Protamex酶水解大豆蛋白的较佳条件 ,并采用凝胶层析过滤色谱分析了不同水解时间水解物分子量的变化 ,发现大豆分离蛋白中存在一些Pro tamex不容易水解成分 ,大豆分离蛋白的Protamex水解过程是一个不均匀的过程。
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| 关 键 词： | 大豆蛋白 多肽 水解 Protamex蛋白酶 |
| 修稿时间： | 2003年10月30 |
Enzymatic Hydrolysis of Soy Protein with Protamex |
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| Zhao Mouming,Wu Jianzhong,Ou Shiyi,Fu Liang,Wang Yong.Enzymatic Hydrolysis of Soy Protein with Protamex[J].Food and Fermentation Industries,2004,30(7):82-86. |
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| Authors: | Zhao Mouming Wu Jianzhong Ou Shiyi Fu Liang Wang Yong |
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| Affiliation: | Zhao Mouming 1 Wu Jianzhong 2 Ou Shiyi 2 Fu Liang 2 Wang Yong 2 1 |
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| Abstract: | The limited hydrolysis process for soy protein with Protamex was studied. And the effects of the main parameters including enzyme substrate ratio, the substrate concentration, pH, hydrolysis time, hydrolysis temperature on hydrolysis reaction were investigated. The optimun hydrolysis condition of soy protein was suggested. The molecular masses of the hydrolysates were determined with Sephadex G 25 gel filtration chromatography.It showed soy protein contains some stable components which are difficult to hydrolyze with Protamex and the process of soy protein hydrolysis is disproportional. |
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| Keywords: | soy protein peptides hydrolysate Protamex |
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